采用离子交换高效液相色谱法结合金电极电化学检测技术快速自动测定血浆和尿液中的D-青霉胺。

Rapid automated determination of D-penicillamine in plasma and urine by ion-exchange high-performance liquid chromatography with electrochemical detection using a gold electrode.

作者信息

Kreuzig F, Frank J

出版信息

J Chromatogr. 1981 Nov 20;218:615-20. doi: 10.1016/s0021-9673(00)82086-5.

DOI:10.1016/s0021-9673(00)82086-5

PMID:7320122

Abstract

A high-performance liquid chromatographic method for the determination of D-penicillamine in plasma and urine has been developed, based on separation on a cation-exchange resin followed by an amperometric detection of the SH group of D-penicillamine oxidized on a gold electrode. The method has been automated and separations from plasma and urine take 7 and 9 min, respectively. The detection limits are 0.05 micrograms/ml in plasma and 0.2 micrograms/ml in urine, with a coefficient of variation of 2.9% (n = 10).

摘要

已开发出一种用于测定血浆和尿液中 D-青霉胺的高效液相色谱法，该方法基于在阳离子交换树脂上进行分离，然后对在金电极上氧化的 D-青霉胺的 SH 基团进行安培检测。该方法已实现自动化，从血浆和尿液中的分离分别需要 7 分钟和 9 分钟。血浆中的检测限为 0.05 微克/毫升，尿液中的检测限为 0.2 微克/毫升，变异系数为 2.9%（n = 10）。