Kreuzig F, Frank J
J Chromatogr. 1981 Nov 20;218:615-20. doi: 10.1016/s0021-9673(00)82086-5.
A high-performance liquid chromatographic method for the determination of D-penicillamine in plasma and urine has been developed, based on separation on a cation-exchange resin followed by an amperometric detection of the SH group of D-penicillamine oxidized on a gold electrode. The method has been automated and separations from plasma and urine take 7 and 9 min, respectively. The detection limits are 0.05 micrograms/ml in plasma and 0.2 micrograms/ml in urine, with a coefficient of variation of 2.9% (n = 10).
已开发出一种用于测定血浆和尿液中 D-青霉胺的高效液相色谱法，该方法基于在阳离子交换树脂上进行分离，然后对在金电极上氧化的 D-青霉胺的 SH 基团进行安培检测。该方法已实现自动化，从血浆和尿液中的分离分别需要 7 分钟和 9 分钟。血浆中的检测限为 0.05 微克/毫升，尿液中的检测限为 0.2 微克/毫升，变异系数为 2.9%（n = 10）。
