大肠杆菌色氨酸操纵子酶的体外合成
In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli.
作者信息
Pouwels P H, Van Rotterdam J
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1786-90. doi: 10.1073/pnas.69.7.1786.
Abstract
Two active enzymes of the tryptophan operon of E. coli, anthranilate synthetase (product of trp E and trp D) and tryptophan synthetase (EC 4.2.1.20) (product of trp B and trp A), have been synthesized in a DNA-dependent preparation for protein synthesis. Anthranilate synthetase and the two components of tryptophan synthetase (tryptophan synthetase A and B) are made in vitro in equimolar amounts, suggesting that regulation of the translation is similar in vivo and in vitro. Kinetics of the synthesis of the two enzymes suggest that transcription and translation proceed in vitro in a correct temporal sequence.
摘要
大肠杆菌色氨酸操纵子的两种活性酶，邻氨基苯甲酸合成酶（trp E和trp D的产物）和色氨酸合成酶（EC 4.2.1.20）（trp B和trp A的产物），已在一种依赖DNA的蛋白质合成制剂中合成。邻氨基苯甲酸合成酶和色氨酸合成酶的两个组分（色氨酸合成酶A和B）在体外以等摩尔量产生，这表明体内和体外的翻译调控相似。这两种酶合成的动力学表明，转录和翻译在体外以正确的时间顺序进行。
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