Kwapinski J B, de Almeida J O, Kwapinski E H
Bull World Health Organ. 1972;46(4):509-13.
Mycobacterium leprae was isolated and purified from lepromas, the spleen, and the liver of leprosy patients. An immunodiffusion analysis of the cytoplasms obtained from four lots of M. leprae and M. lepraemurium, 295 strains of different actinomycetales, and 12 other bacteria was performed with the use of the cytoplasm antisera. Immunological relationships were revealed between the cytoplasms of M. leprae, M. lepraemurium, M. avium, M. gallinarum, M. tuberculosis, M. simiae, M. kansasii, M. chitae, M. capsulatum, Actinomyces israelii, A. naeslundii, and some strains of saprophytic mycobacteria. These studies led to the proposed concept of the immunological evolution of M. leprae and M. lepraemurium and an Actinomyces-like progenitor through M. avium-M. gallinarum and to a proposal for the polyvalent vaccine currently being developed by this research group.
从麻风病人的瘤型病灶、脾脏和肝脏中分离并纯化出麻风分枝杆菌。使用细胞质抗血清，对从四批麻风分枝杆菌和鼠麻风分枝杆菌、295株不同放线菌目菌株以及12种其他细菌中获得的细胞质进行了免疫扩散分析。揭示了麻风分枝杆菌、鼠麻风分枝杆菌、鸟分枝杆菌、鸡分枝杆菌、结核分枝杆菌、猿分枝杆菌、堪萨斯分枝杆菌、希塔分枝杆菌、荚膜分枝杆菌、以色列放线菌、内氏放线菌以及一些腐生性分枝杆菌菌株的细胞质之间的免疫关系。这些研究得出了关于麻风分枝杆菌和鼠麻风分枝杆菌以及通过鸟分枝杆菌-鸡分枝杆菌的类似放线菌祖先的免疫进化的概念，并为本研究小组目前正在研发的多价疫苗提出了建议。
