Kwapinski G, Kwapinski E H
Int J Lepr Other Mycobact Dis. 1976 Oct-Dec;44(4):443-7.
Soluble antigens obtained from purified M. lepraemurium were identified by SDS polyacrylamide gel electrophoresis as three different proteins, M-W 1 X 10(4), 1 X 10(5), and 4 X 10(6), and two polysaccharide-polypeptides. Three antigens were found to react with anti-M. leprae antiserum; a single antigen proved to be immunologically related to M. tuberculosis, M. similae, M. balnei and a few other mycobacteria, but not to Eubacteriales and fungi, as examined by immunoelectrophoresis. In the dermal hypersensitivity assay, the guinea pigs sensitized with soluble antigens of M. lepraemurium only responded to the antigens of M. lepraemurium, M. leprae and partly to M. scrofulaceum, but not to other antigens, including lepromin and leprous serum globulins.
通过SDS聚丙烯酰胺凝胶电泳鉴定，从纯化的鼠麻风杆菌中获得的可溶性抗原为三种不同的蛋白质，分子量分别为1×10⁴、1×10⁵和4×10⁶，以及两种多糖 - 多肽。发现三种抗原与抗麻风杆菌抗血清发生反应；经免疫电泳检测，单一抗原被证明与结核分枝杆菌、类麻风分枝杆菌、浴分支杆菌及其他一些分枝杆菌存在免疫相关性，但与真细菌目和真菌无关。在皮肤超敏反应试验中，用鼠麻风杆菌可溶性抗原致敏的豚鼠仅对鼠麻风杆菌、麻风杆菌的抗原产生反应，部分对瘰疬分枝杆菌的抗原产生反应，但对包括麻风菌素和麻风血清球蛋白在内的其他抗原无反应。
