Edidin M, Weiss A
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2456-9. doi: 10.1073/pnas.69.9.2456.
Reaction of antibody to surface Histocompatibility-2 antigens of cultured mouse fibroblasts causes aggregation of the cellular antigens into caps; these appear as areas of high antigen concentration localized away from the cell processes. Cap formation is inhibited when ATP generation by cells is interrupted, when the temperature is lowered, by addition of cycloheximide-especially over long time periods-and by colcemid. The observations are consistent with a role of the locomotor system of cells in the collection of small antigen-antibody aggregates into a large localized cap. Together with observations on the absence of caps in epithelial cells, these findings suggest that only cells, such as fibroblasts and lymphocytes, that bear leading ruffled membranes are capable of driving antigenantibody aggregates to form caps.
抗体与培养的小鼠成纤维细胞表面组织相容性-2抗原发生反应，会导致细胞抗原聚集形成帽状物；这些帽状物表现为远离细胞突起的高抗原浓度区域。当细胞的ATP生成被中断、温度降低、加入环己酰亚胺（尤其是长时间加入）以及秋水仙酰胺时，帽状物的形成会受到抑制。这些观察结果与细胞运动系统在将小的抗原-抗体聚集体收集成一个大的局部帽状物过程中所起的作用是一致的。连同对上皮细胞中不存在帽状物的观察结果，这些发现表明，只有像成纤维细胞和淋巴细胞这样带有前沿皱膜的细胞才能够促使抗原-抗体聚集体形成帽状物。
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