Cikes M, Friberg S
Proc Natl Acad Sci U S A. 1971 Mar;68(3):566-9. doi: 10.1073/pnas.68.3.566.
A mouse cell line derived from bone marrow (JLS-V9) was infected in vitro with Moloney leukemia virus. After the cell-surface antigens specified by this virus appeared, cells were synchronized in mitosis by a short treatment with colcemid. The expression of H-2- and virus-determined surface antigens was monitored during one cell cycle by an indirect membrane-immunofluorescence test. The highest proportion of antigen-positive cells was found during the G1 period; the proportion dropped as the cells entered the S period, and remained low until they entered the G1 period of the next cycle. The H-2- and virus-determined surface antigens were temporally coexpressed.
一种源自骨髓的小鼠细胞系(JLS-V9)在体外被莫洛尼白血病病毒感染。在这种病毒所特有的细胞表面抗原出现后,通过用秋水仙酰胺进行短暂处理使细胞在有丝分裂中同步化。通过间接膜免疫荧光试验在一个细胞周期内监测H-2和病毒决定的表面抗原的表达。在G1期发现抗原阳性细胞的比例最高;当细胞进入S期时该比例下降,并一直保持较低水平直到进入下一个周期的G1期。H-2和病毒决定的表面抗原在时间上是共表达的。