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H-2和莫洛尼白血病病毒决定的细胞表面抗原在小鼠细胞系同步培养物中的表达。

Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line.

作者信息

Cikes M, Friberg S

出版信息

Proc Natl Acad Sci U S A. 1971 Mar;68(3):566-9. doi: 10.1073/pnas.68.3.566.

Abstract

A mouse cell line derived from bone marrow (JLS-V9) was infected in vitro with Moloney leukemia virus. After the cell-surface antigens specified by this virus appeared, cells were synchronized in mitosis by a short treatment with colcemid. The expression of H-2- and virus-determined surface antigens was monitored during one cell cycle by an indirect membrane-immunofluorescence test. The highest proportion of antigen-positive cells was found during the G1 period; the proportion dropped as the cells entered the S period, and remained low until they entered the G1 period of the next cycle. The H-2- and virus-determined surface antigens were temporally coexpressed.

摘要

一种源自骨髓的小鼠细胞系(JLS-V9)在体外被莫洛尼白血病病毒感染。在这种病毒所特有的细胞表面抗原出现后,通过用秋水仙酰胺进行短暂处理使细胞在有丝分裂中同步化。通过间接膜免疫荧光试验在一个细胞周期内监测H-2和病毒决定的表面抗原的表达。在G1期发现抗原阳性细胞的比例最高;当细胞进入S期时该比例下降,并一直保持较低水平直到进入下一个周期的G1期。H-2和病毒决定的表面抗原在时间上是共表达的。

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