Aist J R, Williams P H
J Cell Biol. 1972 Nov;55(2):368-89. doi: 10.1083/jcb.55.2.368.
Mitosis in Fusarium oxysporum Schlect. was studied by light and electron microscopy. The average times required for the stages of mitosis, as determined from measurements made on living nuclei, were as follows: prophase, 70 sec; metaphase, 120 sec; anaphase, 13 sec; and telophase, 125 sec, for a total of 5.5 min. New postfixation procedures were developed specifically to preserve the fine-structure of the mitotic apparatus. Electron microscopy of mitotic nuclei revealed a fibrillo-granular, extranuclear Spindle Pole Body (SPB) at each pole of the intranuclear, microtubular spindles. Metaphase chromosomes were attached to spindle microtubules via kinetochores, which were found near the spindle poles at telophase. The still-intact, original nuclear envelope constricted around the incipient daughter nuclei during telophase.
通过光学显微镜和电子显微镜对尖孢镰刀菌的有丝分裂进行了研究。根据对活细胞核的测量确定,有丝分裂各阶段所需的平均时间如下:前期70秒;中期120秒;后期13秒;末期125秒,总共5.5分钟。专门开发了新的后固定程序来保存有丝分裂装置的精细结构。有丝分裂细胞核的电子显微镜观察显示,在核内微管纺锤体的每个极点处有一个纤维颗粒状的核外纺锤极体(SPB)。中期染色体通过动粒附着在纺锤体微管上,在末期纺锤极附近发现动粒。在末期,仍然完整的原始核膜围绕着初期子核收缩。