Sulakhe S J, Sulakhe P V
Enzyme. 1979;24(2):137-40. doi: 10.1159/000458642.
A simple, rapid and reliable procedure of tissue preparation was devised to estimate glycogen phosphorylase activity in cardiac and skeletal muscle of normal and genetically dystrophic Syrian hamsters of various ages. Total phosphorylase activities of dystrophic skeletal muscle, compared to normal, were reduced. Except for the case of heart from the younger dystrophic animals (45 days old), in which higher phosphorylase activity was noted, hearts from dystrophic hamsters, compared to normal, also showed reduced phosphorylase activities. There were, however, no significances in the ratios of phosphorylase alpha to total phosphorylase between the normal and dystrophic tissues.
设计了一种简单、快速且可靠的组织制备方法，以评估不同年龄的正常和遗传性营养不良叙利亚仓鼠心脏和骨骼肌中的糖原磷酸化酶活性。与正常情况相比，营养不良骨骼肌的总磷酸化酶活性降低。除了较年轻的营养不良动物（45日龄）心脏中磷酸化酶活性较高外，与正常仓鼠相比，营养不良仓鼠心脏中的磷酸化酶活性也降低。然而，正常组织和营养不良组织之间磷酸化酶α与总磷酸化酶的比率没有显著差异。
