Henderson N S, Tweedle C D, Kabara J J
Neurochem Res. 1980 Dec;5(12):1221-30. doi: 10.1007/BF00964958.
Isozyme patterns of cholinesterase (ChE) from heart, tongue, and skeletal muscle of normal and dystrophic hamsters are presented. Two principal bands, bands 1 and 2, were evaluated. Band 1 migrates faster towards the anode than does band 2. While bands 1 and 2 stain for AChE and were found in control muscles, only band 2 was stained by a pseudocholinesterase (BuChE) and was decreased in samples from dystrophic hamsters. The decrease in BuChE was most pronounced in dystrophic heart muscle. The low level of BuChE measured for dystrophic animal tissue was similar to isozyme patterns found in embryonic tissue and in denervated muscle. BuChE obtained by acrylamide gel electrophoresis along with 16S AchE appears to be a useful biochemical marker of nerve-muscle interactions.
本文展示了正常和营养不良仓鼠心脏、舌头及骨骼肌中胆碱酯酶（ChE）的同工酶模式。评估了两条主要条带，即条带1和条带2。条带1向阳极迁移的速度比条带2快。虽然条带1和条带2对乙酰胆碱酯酶（AChE）染色，且在对照肌肉中发现，但只有条带2被假性胆碱酯酶（BuChE）染色，并且在营养不良仓鼠的样本中减少。BuChE的减少在营养不良的心肌中最为明显。营养不良动物组织中测得的低水平BuChE与胚胎组织和去神经肌肉中发现的同工酶模式相似。通过丙烯酰胺凝胶电泳获得的BuChE与16S乙酰胆碱酯酶（AchE）一起似乎是神经肌肉相互作用的有用生化标志物。
