Avila F R, Schultz R M, Tompkins W A
Infect Immun. 1972 Jul;6(1):9-16. doi: 10.1128/iai.6.1.9-16.1972.
Macrophage immunity to vaccinia virus was examined by measuring replication of vaccinia virus in immune and nonimmune rabbit peritoneal macrophages. Vaccinia virus multiplied in macrophages from normal rabbits but failed to replicate in macrophages from immune rabbits. The specificity of vaccinia restriction was demonstrated by the ability of myxoma virus to replicate in nonimmune and vaccinia-immune macrophages. Vaccinia virus absorbed equally well to immune and nonimmune macrophages, but only a small fraction of immune cells supported synthesis of virus antigen. Virus eclipse and infective center neutralization studies suggested that much of the virus remained at the plasma membrane of immune macrophages, even after prolonged incubation in culture. These data support our previous suggestions that macrophages may contribute to specific immunity against vaccinia virus.
通过检测痘苗病毒在免疫和未免疫兔腹膜巨噬细胞中的复制情况，研究了巨噬细胞对痘苗病毒的免疫作用。痘苗病毒在正常兔的巨噬细胞中增殖，但在免疫兔的巨噬细胞中无法复制。黏液瘤病毒能够在未免疫和痘苗免疫的巨噬细胞中复制，从而证明了痘苗限制的特异性。痘苗病毒与免疫和未免疫的巨噬细胞的吸附效果相同，但只有一小部分免疫细胞支持病毒抗原的合成。病毒隐蔽期和感染中心中和研究表明，即使在培养中长时间孵育后，仍有许多病毒留在免疫巨噬细胞的质膜上。这些数据支持了我们之前的观点，即巨噬细胞可能有助于对痘苗病毒产生特异性免疫。
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