Tompkins W A, Schultz R M
Infect Immun. 1972 Oct;6(4):591-9. doi: 10.1128/iai.6.4.591-599.1972.
Cells cultured from tumors induced in rabbits by inoculation of fibroma virus possessed virus-specific cell surface and cytoplasmic antigens. Tumor cell cultures were capable of a limited number of cell divisions before degenerating. Employing the (51)Cr-release test and the microcytotoxicity test, it was demonstrated that sera from rabbits with regressed fibroma tumors contained antibodies cytotoxic for cells infected in culture with fibroma virus. These sera were only weakly cytotoxic for cultured fibroma tumor cells. In addition, newborn rabbits bearing progressively growing tumors had serum antibodies cytotoxic for cells infected with fibroma virus in culture but not for fibroma tumor cells. Immunofluorescence studies also showed that the reaction of immune serum with the surface antigens of fibroma tumor cells was markedly weaker than with the surface antigens of cells infected with virus in culture. Furthermore, membrane cytotoxicity and immunofluorescence reaction were significantly reduced when cells were tested after prolonged incubation or cell division, or both, following infection with fibroma virus. The failure of tumors to regress in newborn rabbits in spite of the presence of cytotoxic antibodies is discussed in respect to a possible reduction or alteration of virus-specific surface antigens on proliferating tumor cells.
通过接种纤维瘤病毒在兔子体内诱导产生的肿瘤所培养的细胞具有病毒特异性细胞表面和细胞质抗原。肿瘤细胞培养物在退化前能够进行有限次数的细胞分裂。采用铬（51）释放试验和微量细胞毒性试验，证明患有消退性纤维瘤肿瘤的兔子血清中含有对用纤维瘤病毒培养感染的细胞具有细胞毒性的抗体。这些血清对培养的纤维瘤肿瘤细胞仅有微弱的细胞毒性。此外，患有逐渐生长肿瘤的新生兔子血清中具有对用纤维瘤病毒培养感染的细胞而非纤维瘤肿瘤细胞具有细胞毒性的抗体。免疫荧光研究还表明，免疫血清与纤维瘤肿瘤细胞表面抗原的反应明显弱于与培养中感染病毒的细胞表面抗原的反应。此外，在用纤维瘤病毒感染后经过长时间孵育或细胞分裂或两者兼有的情况下对细胞进行检测时，膜细胞毒性和免疫荧光反应显著降低。尽管存在细胞毒性抗体，但新生兔子的肿瘤仍未消退，这一点针对增殖肿瘤细胞上病毒特异性表面抗原可能的减少或改变进行了讨论。
