使用特异性标记的蔗糖来比较口腔链球菌的细胞外葡聚糖和果聚糖代谢。
Use of specifically labeled sucrose for comparison of extracellular glucan and fructan metabolism by oral streptococci.
作者信息
Schachtele C F, Loken A E, Schmitt M K
出版信息
Infect Immun. 1972 Feb;5(2):263-6. doi: 10.1128/iai.5.2.263-266.1972.
Abstract
Sucrose labeled in the fructosyl ((3)H) and glucosyl ((14)C) moieties was used to quantitate extracellular polysaccharide production and degradation by cariogenic and noncariogenic oral streptococci. All of the strains produced glucan and fructan. Streptococcus salivarius produced primarily fructan, whereas S. mutans and S. sanguis produced more glucan than fructan. The cariogenic streptococci could degrade the fructan produced by noncariogenic strains. Although the soluble glucans from all of the strains were sensitive to dextranase, the insoluble glucan from S. mutans could be distinguished from the S. sanguis insoluble glucan by its greater resistance to this enzyme.
摘要
用果糖基（³H）和葡萄糖基（¹⁴C）标记的蔗糖来定量致龋和非致龋口腔链球菌产生和降解细胞外多糖的情况。所有菌株都能产生葡聚糖和果聚糖。唾液链球菌主要产生果聚糖，而变形链球菌和血链球菌产生的葡聚糖比果聚糖多。致龋链球菌能够降解非致龋菌株产生的果聚糖。尽管所有菌株的可溶性葡聚糖对葡聚糖酶敏感，但变形链球菌的不溶性葡聚糖与血链球菌的不溶性葡聚糖相比，对这种酶具有更强的抗性，因此可以区分开来。
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