Forsgren A, Forsum U
Infect Immun. 1972 Apr;5(4):524-30. doi: 10.1128/iai.5.4.524-530.1972.
Of 137 Staphylococcus aureus strains, 87 agglutinated in normal rabbit serum. The agglutination was shown to be caused by the Fc-part of immunoglobulin G (IgG). F(ab(1))(2)-fragments of IgG and immunoglobulin M (IgM) in corresponding concentrations were unreactive. The agglutinating strains had a high or moderate content of protein A. Strains with a low content of protein A and protein A-negative mutants did not agglutinate. The importance of the reaction between the Fc part of IgG and protein A for serotyping of S. aureus is demonstrated. Two alternative methods for serotyping S. aureus are suggested, using either F(ab(1))(2) fragments of IgG or intact IgM.
在137株金黄色葡萄球菌中，87株可被正常兔血清凝集。结果表明，这种凝集是由免疫球蛋白G（IgG）的Fc段引起的。相应浓度的IgG和免疫球蛋白M（IgM）的F(ab(1))(2)片段无反应性。凝集菌株的蛋白A含量高或中等。蛋白A含量低的菌株和蛋白A阴性突变体不发生凝集。证明了IgG的Fc段与蛋白A之间的反应对金黄色葡萄球菌血清分型的重要性。建议了两种用于金黄色葡萄球菌血清分型的替代方法，即使用IgG的F(ab(1))(2)片段或完整的IgM。
