White J G, Conard W J
Am J Pathol. 1973 Jan;70(1):45-56.
The present study has examined the fine structure of freeze-fractured human platelets. Advances in methods used for cell preparation and freezing, together with better instrumentation, have resulted in improved preservation of platelet ultrastructure. Aspects of surface membrane topography and the number, depth of penetration, tortuosity and fenestration of channels belonging to the open canalicular system were revealed in a manner which cannot be fully appreciated in sections of platelets. Elements of the dense tubular system of channels and masses of glycogen were identified in the platelet replicas. The technic may provide an important approach for defining the structural physiology and pathology of blood platelets.
本研究检测了经冷冻断裂处理的人血小板的精细结构。细胞制备和冷冻方法的进步，以及更好的仪器设备，使得血小板超微结构的保存得到了改善。开放小管系统通道的表面膜形貌、数量、穿透深度、曲折度和窗孔等方面得以展现，这是在血小板切片中无法完全观察到的。在血小板复制品中识别出了致密管状系统通道的成分和糖原团块。该技术可能为界定血小板的结构生理学和病理学提供一种重要方法。
