渗透溶血和皂素溶血中膜孔的结构
Structure of membrane holes in osmotic and saponin hemolysis.
作者信息
Seeman P, Cheng D, Iles G H
出版信息
J Cell Biol. 1973 Feb;56(2):519-27. doi: 10.1083/jcb.56.2.519.
Abstract
Serial section electron microscopy of hemolysing erythrocytes (fixed at 12 s after the onset of osmotic hemolysis) revealed long slits and holes in the membrane, extending to around 1 microm in length. Many but not all of the slits and holes (about 100-1000 A wide) were confluent with one another. Ferritin and colloidal gold (added after fixation) only permeated those cells containing membrane defects. No such large holes or slits were seen in saponin-treated erythrocytes, and the membrane was highly invaginated, giving the ghost a scalloped outline. Freeze-etch electron microscopy of saponin-treated membranes revealed 40-50 A-wide pits in the extracellular surface of the membrane. If these pits represent regions from which cholesterol was extracted, then cholesterol is uniformly distributed over the entire erythrocyte membrane.
摘要
对溶血红细胞（在渗透性溶血开始后12秒固定）进行连续切片电子显微镜检查，发现膜上有长裂缝和孔洞，长度延伸至约1微米。许多（但并非全部）裂缝和孔洞（约100 - 1000埃宽）相互汇合。铁蛋白和胶体金（固定后添加）仅渗透到含有膜缺陷的细胞中。在皂素处理的红细胞中未观察到如此大的孔洞或裂缝，并且膜高度内陷，使血影呈现出扇贝状轮廓。对皂素处理的膜进行冷冻蚀刻电子显微镜检查，发现膜的细胞外表面有40 - 50埃宽的凹坑。如果这些凹坑代表胆固醇被提取的区域，那么胆固醇在整个红细胞膜上均匀分布。
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