Patel I B, Vaughn R H
Appl Microbiol. 1973 Jan;25(1):62-9. doi: 10.1128/am.25.1.62-69.1973.
Sloughing spoilage of California ripe olives during processing is characterized by severe softening, skin rupture, and flesh sloughing. It was assumed that cellulolytic activity was responsible for skin rupture and sloughing of flesh, and so a deliberate search was made for cellulolytic bacteria from olives undergoing sloughing spoilage. A bacterium identified as Cellulomonas flavigena was highly cellulolytic, attacking filter paper, carboxymethyl cellulose (CMC) gel, and olive tissue. Other bacteria attacking CMC, but not filter paper, enhanced the activity of the Cellulomonas strain when grown in mixed culture, although they did not, in pure culture, have any effect on filter paper. These latter cultures (all degraded olive tissue) represented the genera Xanthomonas, Aerobacter, and Escherichia. Other noncellulolytic bacteria belonging to the genera Alcaligenes, Kurthia, and Micrococcus also were used for study of mixed culture fermentation of cellulose by C. flavigena. Cellobiose accumulation at levels of 1.0% (w/v) and above suppressed growth of C. flavigena.
加利福尼亚成熟橄榄在加工过程中的脱落腐败表现为严重软化、果皮破裂和果肉脱落。据推测，纤维素分解活性是导致果皮破裂和果肉脱落的原因，因此人们特意从正在经历脱落腐败的橄榄中寻找纤维素分解细菌。一种被鉴定为黄纤维单胞菌的细菌具有高度纤维素分解能力，能分解滤纸、羧甲基纤维素（CMC）凝胶和橄榄组织。其他能分解CMC但不能分解滤纸的细菌，在混合培养时会增强纤维单胞菌菌株的活性，尽管它们在纯培养时对滤纸没有任何影响。这些后来的培养物（均能降解橄榄组织）分别属于黄单胞菌属、气杆菌属和大肠杆菌属。其他属于产碱菌属、库尔希菌属和微球菌属的非纤维素分解细菌也被用于研究黄纤维单胞菌对纤维素的混合培养发酵。纤维二糖积累量达到1.0%（w/v）及以上时会抑制黄纤维单胞菌的生长。
