多胺限制条件下大肠杆菌突变体的生长及大分子组成
Growth and macromolecular composition of a mutant of Escherichia coli during polyamine limitation.
作者信息
Morris D R, Jorstad C M
出版信息
J Bacteriol. 1973 Jan;113(1):271-7. doi: 10.1128/jb.113.1.271-277.1973.
Abstract
A mutant of Escherichia coli with reduced levels of biosynthetic arginine decarboxylase was isolated which required putrescine or spermidine for optimal growth. The stimulation of growth by putrescine was 1.5- to 3-fold depending upon the culture medium. Specificity studies supported the concept that the requirement was for spermidine or closely related polyamines, or for diamines which could be converted enzymatically to these compounds. The behavior of the macromolecular composition of the polyamine-starved cells appeared abnormal. The ribonucleic acid to protein and deoxyribonucleic acid to cell ratios in the starved cells were both higher than expected on the basis of their growth rate. The stable ribonucleic acid in the polyamine-limited cells appeared to be normal as judged from size distribution and degree of methylation. The relationship of these results to mechanisms for regulation of nucleic acid and protein synthesis in E. coli is discussed.
摘要
分离出一种生物合成精氨酸脱羧酶水平降低的大肠杆菌突变体，该突变体需要腐胺或亚精胺才能实现最佳生长。根据培养基的不同，腐胺对生长的刺激作用为1.5至3倍。特异性研究支持了这样一种观点，即所需的是亚精胺或密切相关的多胺，或者是可以通过酶促转化为这些化合物的二胺。多胺饥饿细胞的大分子组成表现异常。饥饿细胞中的核糖核酸与蛋白质之比以及脱氧核糖核酸与细胞之比均高于根据其生长速率预期的值。从大小分布和甲基化程度判断，多胺受限细胞中的稳定核糖核酸似乎正常。本文讨论了这些结果与大肠杆菌核酸和蛋白质合成调控机制的关系。
相似文献
1
Growth and macromolecular composition of a mutant of Escherichia coli during polyamine limitation.多胺限制条件下大肠杆菌突变体的生长及大分子组成
J Bacteriol. 1973 Jan;113(1):271-7. doi: 10.1128/jb.113.1.271-277.1973.
2
Polyamine metabolism in potassium-deficient bacteria.缺钾细菌中的多胺代谢
J Bacteriol. 1972 Dec;112(3):1213-21. doi: 10.1128/jb.112.3.1213-1221.1972.
3
Polyamines in the synthesis of bacteriophage deoxyribonucleic acid. II. Requirement for polyamines in T4 infection of a polyamine auxotroph.多胺在噬菌体脱氧核糖核酸合成中的作用。II. 多胺营养缺陷型菌株T4感染中对多胺的需求
J Virol. 1972 Mar;9(3):423-30. doi: 10.1128/JVI.9.3.423-430.1972.
4
Regulation of macromolecular synthesis by putrescine in a conditional Escherichia coli putrescine auxotroph.腐胺对条件性大肠杆菌腐胺营养缺陷型中大分子合成的调控
J Bacteriol. 1972 Oct;112(1):30-9. doi: 10.1128/jb.112.1.30-39.1972.
5
Polyamines and the accumulation of ribonucleic acid in some polyauxotrophic strains of Escherichia coli.多胺与大肠杆菌某些多营养缺陷型菌株中核糖核酸的积累
J Bacteriol. 1967 Nov;94(5):1684-96. doi: 10.1128/jb.94.5.1684-1696.1967.
6
Polyamine requirements of a conditional polyamine auxotroph of Escherichia coli.大肠杆菌条件性多胺营养缺陷型对多胺的需求
J Bacteriol. 1973 Aug;115(2):469-75. doi: 10.1128/jb.115.2.469-475.1973.
7
Polyamine stimulation of nucleic acid synthesis in an uninfected and phage-infected polyamine auxotroph of Escherichia coli K12 (arginine-agmatine ureohydrolase-putrescine-spermidine-lysine-cadaverine).多胺对大肠杆菌K12的未感染及噬菌体感染的多胺营养缺陷型（精氨酸-胍丁胺脲水解酶-腐胺-亚精胺-赖氨酸-尸胺）核酸合成的刺激作用
Proc Natl Acad Sci U S A. 1972 Jan;69(1):213-7. doi: 10.1073/pnas.69.1.213.
8
Polyamine regulation of stringent control in a polyamine-auxotrophic strain of Escherichia coli.多胺对大肠杆菌多胺营养缺陷型菌株严谨控制的调节作用
Biochem J. 1984 Apr 1;219(1):205-10. doi: 10.1042/bj2190205.
9
Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase.鸟氨酸脱羧酶合成受阻的大肠杆菌突变体的分离、特性鉴定及定位
J Bacteriol. 1975 Nov;124(2):791-9. doi: 10.1128/jb.124.2.791-799.1975.
10
Adjustment of polyamine contents in Escherichia coli.大肠杆菌中多胺含量的调节
J Bacteriol. 1988 Jul;170(7):3131-5. doi: 10.1128/jb.170.7.3131-3135.1988.
引用本文的文献
1
Polyamine blockade and binding energetics in the MthK potassium channel.多胺阻断和 MthK 钾通道的结合能。
J Gen Physiol. 2020 Jul 6;152(7). doi: 10.1085/jgp.201912527.
2
Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii.鲍曼不动杆菌中参与1,3 -二氨基丙烷产生途径的L-2,4-二氨基丁酸：2-酮戊二酸4-氨基转移酶编码基因的鉴定与分析。
J Bacteriol. 1997 Aug;179(16):5118-25. doi: 10.1128/jb.179.16.5118-5125.1997.
3
Stimulation of deoxyribonucleic acid replication fork movement by spermidine analogs in polyamine-deficient Escherichia coli.在多胺缺乏的大肠杆菌中，亚精胺类似物对脱氧核糖核酸复制叉移动的刺激作用。
J Bacteriol. 1980 Mar;141(3):1192-8. doi: 10.1128/jb.141.3.1192-1198.1980.
4
Reversible inhibition of bacterial growth after specific inhibition of spermidine synthase by dicyclohexylamine.二环己胺特异性抑制亚精胺合酶后细菌生长的可逆抑制
Biochem J. 1984 Nov 1;223(3):823-30. doi: 10.1042/bj2230823.
5
Localization of polyamine enhancement of protein synthesis to subcellular components of Escherichia coli and Pseudomonas sp. strain Kim.多胺对蛋白质合成的促进作用在大肠杆菌和假单胞菌属金氏菌株亚细胞组分中的定位
J Bacteriol. 1983 Jan;153(1):326-34. doi: 10.1128/jb.153.1.326-334.1983.
6
Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.通过用DNA回旋酶抑制剂处理大肠杆菌可产生正超螺旋质粒DNA。
Nucleic Acids Res. 1983 May 25;11(10):2999-3017. doi: 10.1093/nar/11.10.2999.
7
Structural specificity of the spermidine requirement of an Escherichia coli auxotroph.大肠杆菌营养缺陷型对亚精胺需求的结构特异性
J Bacteriol. 1980 Feb;141(2):456-63. doi: 10.1128/jb.141.2.456-463.1980.
8
Polyamine limitation of growth slows the rate of polypeptide chain elongation in Escherichia coli.多胺对生长的限制会减缓大肠杆菌中多肽链延伸的速率。
J Bacteriol. 1974 Sep;119(3):857-60. doi: 10.1128/jb.119.3.857-860.1974.
9
Influence of polyamine limitation on the chain growth rates of beta-galactosidase and of its messenger ribonucleic acid.多胺限制对β-半乳糖苷酶及其信使核糖核酸链生长速率的影响。
J Bacteriol. 1973 Nov;116(2):588-92. doi: 10.1128/jb.116.2.588-592.1973.
10
Polyamines in microorganisms.微生物中的多胺
Microbiol Rev. 1985 Mar;49(1):81-99. doi: 10.1128/mr.49.1.81-99.1985.
本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
SPERMIDINE, SPERMINE, AND RELATED AMINES.亚精胺、精胺及相关胺类
Pharmacol Rev. 1964 Sep;16:245-300.
4
Selecting bacterial mutants by the penicillin method.用青霉素法筛选细菌突变体。
Science. 1960 Feb 26;131(3400):604-5. doi: 10.1126/science.131.3400.604.
5
The acetylation of polyamines in Escherichia coli.大肠杆菌中多胺的乙酰化作用。
J Biol Chem. 1960 Mar;235:776-82.
6
Control by endogenously synthesized arginine of the formation of ornithine transcarbamylase in Escherichia coli.大肠杆菌中内源性合成的精氨酸对鸟氨酸转氨甲酰酶形成的调控。
J Bacteriol. 1961 Feb;81(2):236-40. doi: 10.1128/jb.81.2.236-240.1961.
7
The biosynthesis of spermidine and spermine from putrescine and methionine.由腐胺和蛋氨酸生物合成亚精胺和精胺。
J Biol Chem. 1958 Oct;233(4):907-14.
8
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
9
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
10
Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离多种核糖核酸种类
Biochemistry. 1967 Jun;6(6):1818-27. doi: 10.1021/bi00858a033.
Zotero 插件