Jorstad C M, Harada J J, Morris D R
J Bacteriol. 1980 Feb;141(2):456-63. doi: 10.1128/jb.141.2.456-463.1980.
A homologous series of spermidine analogs was synthesized with the general structure NH3+ (CH2)nNH2+(CH2)3NH3+, where spermidine has n = 4. The influence of these compounds on growth and on the syntheses of protein and messenger ribonucleic acid was examined in a spermidine auxotroph of Escherichia coli. All of the homologs tested were taken up by the cells to an intracellular level equivalent to the level of spermidine which gives optimal growth. With increasing chain length of the homologs, there was reduced ability to stimulate growth. The homologs with n = 7 and n = 8 were essentially inactive. A similar specificity was observed when the ability of the homologs to restore the rates of protein and messenger ribonucleic acid chain elongation was compared to that of spermidine. These results suggest that a definite spatial arrangement of the amino groups of spermidine is required for productive interaction at its intracellular site(s) of action.
合成了一系列具有通式NH3+(CH2)nNH2+(CH2)3NH3+的亚精胺类似物，其中亚精胺的n = 4。在大肠杆菌的一个亚精胺营养缺陷型菌株中，研究了这些化合物对生长以及蛋白质和信使核糖核酸合成的影响。所有测试的同系物都被细胞摄取到与能实现最佳生长的亚精胺水平相当的细胞内浓度。随着同系物链长的增加，刺激生长的能力降低。n = 7和n = 8的同系物基本无活性。当将同系物恢复蛋白质和信使核糖核酸链延伸速率的能力与亚精胺的能力进行比较时，观察到了类似的特异性。这些结果表明，亚精胺氨基的特定空间排列是其在细胞内作用位点进行有效相互作用所必需的。
