Hobbs J B, Cliff W J
J Exp Med. 1973 Mar 1;137(3):776-98. doi: 10.1084/jem.137.3.776.
When portions of adult renal tissue are allografted into the rabbit ear chamber, they usually survive for periods of up to several months (6). When a kidney from the same donor is grafted as a whole organ, the ear chamber grafts then reject with the whole organ in 7 days. During that time serial needle biopsies of the whole organ are compared with the in vivo appearance of the ear chamber grafts. This establishes that the changes occurring in the ear chamber grafts are monitoring the rejection process proceeding in the whole organ grafts. Dramatic vascular changes herald the earliest stages of unmodified rejection. A highly characteristic form of individual discrete platelet adhesion to both endothelium and adherent leukocytes is observed which is associated with the release reaction. At times as many as 20 such discrete platelets are clearly visible in profile in one high-power field. This demonstrates in vivo a mechanism whereby vascular and parenchymal damage may be produced by platelet contents, without previous aggregation or thrombus formation being necessary.
当将成年肾脏组织的部分移植到兔耳腔时，它们通常能存活长达数月（6个月）。当将来自同一供体的肾脏作为一个完整器官进行移植时，耳腔移植组织会在7天内与整个器官一起发生排斥反应。在此期间，对整个器官进行系列针吸活检，并与耳腔移植组织的体内外观进行比较。这证实了耳腔移植组织中发生的变化正在监测整个器官移植中进行的排斥过程。显著的血管变化预示着未修饰排斥反应的最早阶段。观察到一种高度特征性的单个离散血小板同时粘附于内皮细胞和粘附白细胞的形式，这与释放反应相关。有时在一个高倍视野中可以清楚地看到多达20个这样的离散血小板的轮廓。这在体内证明了一种机制，即血小板内容物可能导致血管和实质损伤，而无需先前的聚集或血栓形成。
