Paetzke-Brunner I, Löffler G, Wieland O H
Horm Metab Res. 1979 Apr;11(4):285-8.
Isolated fat cells from rat brown adipose tissue in vitro respond to insulin with an increase of pyruvate dehydrogenase (EC 1.2.4.1) activity due to conversion of the inactive form of the enzyme (PDHb) to the active form (PDHa). Like in white adipocytes this effect depends on the presence of glucose or 2-deoxyglucose in the medium. The interrelationship between the steady state of the PDH-system and the phosphorylation state of the adenine nucleotides was studied in white adipose tissue. While insulin in the presence of 2-deoxyglucose caused a large fall of the tissue ATP/ADP ratio which could explain the increase of PDHa activity, the ATP/ADP ratio remained unchanged during incubations with insulin and glucose. Thus it appears that other factors than the ATP/ADP ratio are involved in the regulation of PDH activity by insulin the nature of which remains to be elucidated.
体外培养的大鼠棕色脂肪组织分离脂肪细胞对胰岛素产生反应，丙酮酸脱氢酶（EC 1.2.4.1）活性增加，这是由于该酶的无活性形式（PDHb）转化为活性形式（PDHa）。与白色脂肪细胞一样，这种效应取决于培养基中葡萄糖或2-脱氧葡萄糖的存在。在白色脂肪组织中研究了PDH系统的稳态与腺嘌呤核苷酸磷酸化状态之间的相互关系。虽然在2-脱氧葡萄糖存在下胰岛素导致组织ATP/ADP比值大幅下降，这可以解释PDHa活性的增加，但在胰岛素和葡萄糖孵育期间，ATP/ADP比值保持不变。因此，似乎除了ATP/ADP比值之外，还有其他因素参与胰岛素对PDH活性的调节，其本质仍有待阐明。
