大肠杆菌中存在两种精氨酰 - 转移核糖核酸合成酶活性的证据。
Evidence for the existence of two arginyl-transfer ribonucleic acid synthetase activities in Escherichia coli.
作者信息
Yem D W, Williams L S
出版信息
J Bacteriol. 1973 Feb;113(2):891-4. doi: 10.1128/jb.113.2.891-894.1973.
Abstract
Two arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: ribonucleic acid ligase adenosine monophosphate) activities were found in extracts of Escherichia coli strains AB1132 and NP2. The two arginyl-tRNA synthetase activities in extracts of strain AB1132 were found to be separable by diethylaminoethyl-cellulose column chromatography, Sephadex column fractionation, and by sucrose density gradient centrifugation. In addition, in the standard assay using extracts of strain AB1132 there were two pH optima for arginyl-tRNA synthetase activity. Furthermore, when arginyl-tRNA synthetase of strain NP2 was fractionated by hydroxylapatite column chromatography, two activities were observed which were similar to those of strain AB1132.
摘要
在大肠杆菌菌株AB1132和NP2的提取物中发现了两种精氨酰转移核糖核酸（tRNA）合成酶（EC 6.1.1.13，精氨酸：核糖核酸连接酶单磷酸腺苷）活性。发现菌株AB1132提取物中的两种精氨酰-tRNA合成酶活性可通过二乙氨基乙基纤维素柱色谱、葡聚糖柱分级分离和蔗糖密度梯度离心分离。此外，在使用菌株AB1132提取物的标准测定中，精氨酰-tRNA合成酶活性有两个最适pH值。此外，当菌株NP2的精氨酰-tRNA合成酶通过羟基磷灰石柱色谱分级分离时，观察到两种活性，它们与菌株AB1132的活性相似。
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