大肠杆菌中精氨酸生物合成的调控：精氨酰转移核糖核酸合成酶突变体的特性分析
Control of arginine biosynthesis in Escherichia coli: characterization of arginyl-transfer ribonucleic acid synthetase mutants.
作者信息
Williams A L, Williams L S
出版信息
J Bacteriol. 1973 Mar;113(3):1433-41. doi: 10.1128/jb.113.3.1433-1441.1973.
Abstract
The arginyl-transfer ribonucleic acid (Arg-tRNA) synthetase (EC 6.1.1.13, arginine: RNA ligase adenosine monophosphate) mutants, exhibiting nonrepressible synthesis of arginine by exogenous arginine, were employed in studies of several biochemical properties. Two of these mutants possessed Arg-tRNA synthetases with a reduced affinity for arginine, and this enzyme of another mutant had a reduced affinity for arginine-tRNA (tRNA(arg)). The mutant possessing an Arg-tRNA synthetase with an altered K(m) for tRNA(arg) was found to have reduced in vivo aminoacylation of two of the five isoaccepting species of tRNA(arg) and complete absence of aminoacylation of one of the isoaccepting species.
摘要
精氨酰转移核糖核酸（Arg-tRNA）合成酶（EC 6.1.1.13，精氨酸：RNA连接酶单磷酸腺苷）突变体，表现出对外源精氨酸不可抑制的精氨酸合成，被用于多项生化特性研究。这些突变体中的两个具有对精氨酸亲和力降低的Arg-tRNA合成酶，另一个突变体的这种酶对精氨酰-tRNA（tRNA(arg)）的亲和力降低。发现拥有对tRNA(arg)的K(m)改变的Arg-tRNA合成酶的突变体，其体内五种同功受体类型的tRNA(arg)中有两种的氨酰化作用降低，且有一种同功受体类型完全没有氨酰化作用。
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Control of arginine biosynthesis in Escherichia coli: characterization of arginyl-transfer ribonucleic acid synthetase mutants.大肠杆菌中精氨酸生物合成的调控：精氨酰转移核糖核酸合成酶突变体的特性分析
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Biochemical and genetic characterization of a mutant of Escherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase.具有温度敏感性缬氨酰核糖核酸合成酶的大肠杆菌突变体的生化与遗传特性
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Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. IV. Further studies on the role of arginine transfer RNA repression of the enzymes of arginine biosynthesis.大肠杆菌中精氨酸生物合成抑制机制的研究。IV. 关于精氨酸转移RNA对精氨酸生物合成酶抑制作用的进一步研究。
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Mutants of Salmonella typhimurium with an altered leucyl-transfer ribonucleic acid synthetase.鼠伤寒沙门氏菌中亮氨酰转运核糖核酸合成酶发生改变的突变体。
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Binding of transfer RNA and arginine to the arginine transfer RNA synthetase of Escherichia coli.转运RNA和精氨酸与大肠杆菌精氨酸转运RNA合成酶的结合。
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