牛衣原体流产的被动血凝试验
Passive hemagglutination test for bovine chlamydial abortion.
作者信息
Belden E L, McKercher D G
出版信息
Infect Immun. 1973 Feb;7(2):141-6. doi: 10.1128/iai.7.2.141-146.1973.
Abstract
Purified chlamydial elementary bodies were subjected to sonic lysis to produce soluble antigen for passive hemagglutination (PHA). PHA was more sensitive than complement fixation (CF) in detecting antibody in hyperimmunized rabbits, lambs, and calves. Both CF and PHA tests were used to monitor antibody responses in experimentally infected pregnant cows. All animals responded with rises in titers after experimental infection. Titers declined and then increased a second time near the time of abortion. PHA titers were considerably higher than CF titers.
摘要
将纯化的衣原体原体进行超声裂解，以产生用于被动血凝试验（PHA）的可溶性抗原。在检测经高度免疫的兔、羔羊和犊牛的抗体时，PHA比补体结合试验（CF）更敏感。CF和PHA试验均用于监测实验感染的怀孕母牛的抗体反应。所有动物在实验感染后滴度均升高。滴度下降，然后在流产时再次升高。PHA滴度明显高于CF滴度。
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