大肠杆菌K-12接合过程中的生理学：与致死合子形成相关的受体细胞功能改变
Physiology of Escherichia coli K-12 during conjugation: altered recipient cell functions associated with lethal zygosis.
作者信息
Skurray R A, Reeves P
出版信息
J Bacteriol. 1973 Apr;114(1):11-7. doi: 10.1128/jb.114.1.11-17.1973.
Abstract
The number of viable F(-) cells decreases when Escherichia coli recipient cells are mixed with an excess of Hfr cells. Evidence is presented showing that lethal zygosis was accompanied by changes in the physiology of the recipient cells, including (i) inhibition of deoxyribonucleic acid synthesis, (ii) inhibition of beta-galactosidase induction, (iii) altered transport and accumulation of galactosides, and (iv) leakage of beta-galactosidase into the supernatant fluid. The results are discussed in terms of possible conjugation-associated changes that, at high Hfr to F(-) ratios, lead to lethal zygosis.
摘要
当大肠杆菌受体细胞与过量的高频重组（Hfr）细胞混合时，存活的F(-)细胞数量会减少。有证据表明，致死性合子形成伴随着受体细胞生理变化，包括：（i）脱氧核糖核酸合成受到抑制；（ii）β-半乳糖苷酶诱导受到抑制；（iii）半乳糖苷的转运和积累发生改变；（iv）β-半乳糖苷酶泄漏到上清液中。本文根据高Hfr与F(-)比例下可能导致致死性合子形成的接合相关变化对结果进行了讨论。
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