在接合以及单链脱氧核糖核酸噬菌体吸附方面存在缺陷的大肠杆菌雌性菌株。
Escherichia coli females defective in conjugation and in adsorption of a single-stranded deoxyribonucleic acid phage.
作者信息
Reiner A M
出版信息
J Bacteriol. 1974 Jul;119(1):183-91. doi: 10.1128/jb.119.1.183-191.1974.
Abstract
We predicted that, among mutants resistant to infection by single-stranded deoxyribonucleic acid viruses, there would be some also resistant to "infection" by single-stranded conjugal deoxyribonucleic acid. Approximately 5% of the Escherichia coli K-12 females selected for resistance to phage ST-1 were defective as recipients in conjugation. These spontaneous mutants fell into two classes. Type A accepted both plasmid and chromosomal markers at greatly reduced frequencies (<10(-6) of normal for at least one strain), formed "rough" colonies, and (unlike their parent) were nonflagellated. Type B strains accepted both chromosomal and plasmid markers at reduced frequencies (10(-2) to 10(-1) of normal), were temperature sensitive for growth, and showed increased susceptibility towards antibiotics and deoxycholate. Both classes of mutants also were resistant to certain female-specific viruses.
摘要
我们预测，在对单链脱氧核糖核酸病毒感染具有抗性的突变体中，会有一些对单链接合脱氧核糖核酸的“感染”也具有抗性。在被选择为对噬菌体ST-1具有抗性的大肠杆菌K-12雌性菌株中，约5%作为接合受体存在缺陷。这些自发突变体分为两类。A类以大大降低的频率（至少对一个菌株而言低于正常频率的10^(-6)）接受质粒和染色体标记，形成“粗糙”菌落，并且（与它们的亲本不同）无鞭毛。B类菌株以降低的频率（正常频率的10^(-2)至10^(-1)）接受染色体和质粒标记，对生长温度敏感，并且对抗生素和脱氧胆酸盐的敏感性增加。这两类突变体对某些雌性特异性病毒也具有抗性。
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