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基于包涵体形态以及对二乙氨基乙基葡聚糖和放线菌酮的反应对鹦鹉热衣原体进行生物分型。

Biotyping of Chlamydia psittaci based on inclusion morphology and response to diethylaminoethyl-dextran and cycloheximide.

作者信息

Spears P, Storz J

出版信息

Infect Immun. 1979 Apr;24(1):224-32. doi: 10.1128/iai.24.1.224-232.1979.

DOI:10.1128/iai.24.1.224-232.1979
PMID:457272
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC414287/
Abstract

Strains of Chlamydia psittaci from cattle, sheep, pigs, mice, guinea pigs, rabbits, cats, and parrots were subdivided based on their biological characteristics. Chlamydiae grown in the yolk sac of chicken embryos were used to infect L cell monolayers. The host cells were infected without further treatment or treated with diethylaminoethyl-dextran, cycloheximide, or both. The following criteria were used for biotyping the strains: the morphology of the inclusions and time after infection at which they appeared, the effect of chlamydial multiplication on the host cell cytoskeleton, and the change in the number of cells infected in response to diethylaminoethyl-dextran and cycloheximide. These properties were determined for 29 strains of C. psittaci. Based on the results, the strains were placed into eight biotypes.

摘要

根据生物学特性，对来自牛、羊、猪、小鼠、豚鼠、兔子、猫和鹦鹉的鹦鹉热衣原体菌株进行了细分。在鸡胚卵黄囊中生长的衣原体用于感染L细胞单层。宿主细胞在未经进一步处理或用二乙氨基乙基葡聚糖、放线菌酮或两者处理后被感染。以下标准用于对菌株进行生物分型：包涵体的形态及其出现后的感染时间、衣原体增殖对宿主细胞细胞骨架的影响，以及响应二乙氨基乙基葡聚糖和放线菌酮而感染的细胞数量变化。对29株鹦鹉热衣原体菌株测定了这些特性。根据结果，这些菌株被分为八个生物型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/42379e08606a/iai00184-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/a561bc2e08ef/iai00184-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/f284b1e436e3/iai00184-0240-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/269423f95d40/iai00184-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/bda4493d65c7/iai00184-0241-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/b61885f7cfc4/iai00184-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/673147a50349/iai00184-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/ba13d555a108/iai00184-0243-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/42379e08606a/iai00184-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/a561bc2e08ef/iai00184-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/f284b1e436e3/iai00184-0240-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/269423f95d40/iai00184-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/bda4493d65c7/iai00184-0241-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/b61885f7cfc4/iai00184-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/673147a50349/iai00184-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/ba13d555a108/iai00184-0243-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7947/414287/42379e08606a/iai00184-0244-a.jpg

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