Satija K C, Rajpal S, Pandey R, Sharma V K
Infect Immun. 1979 May;24(2):567-70. doi: 10.1128/iai.24.2.567-570.1979.
Antigenic components of buffalo (Bos bubalis) serum, which were also components of buffalo colostrum, seminal plasma, milk whey, saliva, and tears, were investigated by the ager gel diffusion test and immunoelectrophoresis. Immunoglobulins of buffalo serum were identified by immunoelectrophoresis employing rabbit-anti-buffalo serum and rabbit-anti-buffalo gamma-globulin. Based on immunoelectrophoretic patterns immunoglobulin G (IgG), IgGA, and IgM were detected both in the serum and colostrum of buffaloes. Tears contained both IgG and IgM. Cross-reactions of buffalo serum with seminal plasma, saliva, and milk whey were observed only in the IgG region. By polyacrylamide gel electrophoresis, lipoprotein (5.2% +/- 0.41), IgM (11.4% +/- 3.1), IgG (9.4% +/- 0.98), haptoglobin 21.8% +/- 3.73), transferrin (10.4% +/- 2.15), ceruloplasmin (7.8% +/- 1.3), postalbumin (20.8% +/- 2.09), and albumin (13.7% +/- 0.75) were identified provisionally.
采用琼脂凝胶扩散试验和免疫电泳法，对水牛血清中的抗原成分进行了研究，这些抗原成分也是水牛初乳、精浆、乳清、唾液和泪液中的成分。采用兔抗水牛血清和兔抗水牛γ球蛋白，通过免疫电泳鉴定了水牛血清中的免疫球蛋白。根据免疫电泳图谱，在水牛血清和初乳中均检测到免疫球蛋白G（IgG）、IgGA和IgM。泪液中同时含有IgG和IgM。仅在IgG区域观察到水牛血清与精浆、唾液和乳清的交叉反应。通过聚丙烯酰胺凝胶电泳，初步鉴定出脂蛋白（5.2%±0.41）、IgM（11.4%±3.1）、IgG（9.4%±0.98）、触珠蛋白（21.8%±3.73）、转铁蛋白（10.4%±2.15）、铜蓝蛋白（7.8%±1.3）、后白蛋白（20.8%±2.09）和白蛋白（13.7%±0.75）。
