Baker R J, Valli V E
Department of Pathology, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1988 Jul;52(3):308-14.
Serum from 28 clinically healthy cats was subjected to agarose gel electrophoresis and the migration distance relative to albumin was determined. The reference values for the relative and absolute concentrations of each protein fraction were determined and compared to previous reports. The immunoelectrophoretic, crossed immunoelectrophoretic and crossed line immunoelectrophoretic pattern, of a pooled sample of serum from clinically normal cats was determined. The cross-reactivity between goat and/or rabbit monospecific antisera to human proteins and feline serum was determined using immunoelectrophoresis and crossed-line absorption immunoelectrophoresis. Feline alpha-2-macroglobulin, haptoglobin, B1C-globulin, IgG, albumin and ceruloplasmin cross reacted strongly with the monospecific antisera. Alpha-2-macroglobulin migrated anodal to haptoglobin. Lipoproteins and ceruloplasmin were studied using staining procedures described in man. Feline transferrin was precipitated with Rivanol.
对28只临床健康猫的血清进行琼脂糖凝胶电泳，并测定各蛋白组分相对于白蛋白的迁移距离。确定了各蛋白组分相对浓度和绝对浓度的参考值，并与以往报告进行比较。测定了临床正常猫血清混合样本的免疫电泳、交叉免疫电泳和交叉线免疫电泳图谱。使用免疫电泳和交叉线吸收免疫电泳测定山羊和/或兔抗人蛋白单特异性抗血清与猫血清之间的交叉反应性。猫α-2-巨球蛋白、触珠蛋白、B1C球蛋白、IgG、白蛋白和铜蓝蛋白与单特异性抗血清强烈交叉反应。α-2-巨球蛋白向阳极迁移至触珠蛋白。使用人类描述的染色程序研究脂蛋白和铜蓝蛋白。用利凡诺沉淀猫转铁蛋白。
