噬菌体尾部成分。V. 用蝶酰六谷氨酸对T4D基因28感染的细菌提取物进行互补作用。
Bacteriophage tail components. V. Complementation of T4D gene 28 - -infected bacterial extracts with pteroyl hexaglutamate.
作者信息
Kozloff L M, Lute M, Baugh C M
出版信息
J Virol. 1973 May;11(5):637-41. doi: 10.1128/JVI.11.5.637-641.1973.
Abstract
Synthetic pteroyl hexaglutamate (9 x 10(-6) M) stimulated the formation of new T4D particles in vitro in extracts of Escherichia coli B infected with T4D gene 28(-). The stimulation was specific for this form of folic acid since neither pteroyl pentaglutamate nor pteroyl heptaglutamate stimulated phage formation. T4D formation in vitro in E. coli B extracts prepared after infection with 11 other phage mutants known to be involved in phage tail plate formation (5(-), 6(-), 7(-), 8(-), 10(-), 25(-), 26(-), 27(-), 29(-), 51(-), 53(-)) was not stimulated by the addition of pteroyl hexaglutamate. It can be concluded that the T4D gene 28 product is involved in the formation of the phage tail plate pteroyl hexaglutamate.
摘要
合成的蝶酰六谷氨酸（9×10⁻⁶ M）在体外刺激了用T4D基因28⁻感染的大肠杆菌B提取物中新型T4D颗粒的形成。这种刺激对这种叶酸形式具有特异性，因为蝶酰五谷氨酸和蝶酰七谷氨酸均未刺激噬菌体形成。在用已知参与噬菌体尾板形成的其他11种噬菌体突变体（5⁻、6⁻、7⁻、8⁻、10⁻、25⁻、26⁻、27⁻、29⁻、51⁻、53⁻）感染后制备的大肠杆菌B提取物中，体外T4D的形成不会因添加蝶酰六谷氨酸而受到刺激。可以得出结论，T4D基因28产物参与噬菌体尾板蝶酰六谷氨酸的形成。
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