Kozloff L M, Lute M, Crosby L K
J Virol. 1970 Dec;6(6):754-9. doi: 10.1128/JVI.6.6.754-759.1970.
The assembly of T4D tail plates occurring during in vitro complementation reactions was found to be stimulated by pteroyl hexaglutamate. Neither the pteroyl pentaglutamate nor the pteroyl heptaglutamate substituted for the hexaglutamate. A small stimulation of the rate and amount of T4D tail plate assembly was observed in untreated extracts. A greater stimulation occurred when activated charcoal-treated bacterial extracts were used. Charcoal treatment inhibited complementation only when no preformed tail plates were present in the extracts, and the inhibition was reversed by the addition of 9 x 10(-6)m chemically synthesized pteroyl hexaglutamate. The stimulation is apparently due to a requirement for the pteroyl hexaglutamate for tail plate assembly.
发现在体外互补反应过程中，四型噬菌体（T4D）尾板的组装受到蝶酰六谷氨酸的刺激。蝶酰五谷氨酸和蝶酰七谷氨酸都不能替代六谷氨酸。在未处理的提取物中观察到T4D尾板组装速率和数量有轻微刺激。当使用经活性炭处理的细菌提取物时，刺激作用更强。活性炭处理仅在提取物中不存在预先形成的尾板时才抑制互补作用，并且通过添加9×10⁻⁶m化学合成的蝶酰六谷氨酸可逆转这种抑制作用。这种刺激显然是由于尾板组装需要蝶酰六谷氨酸。
