Cooper S, Ruettinger T
J Bacteriol. 1973 Jun;114(3):966-73. doi: 10.1128/jb.114.3.966-973.1973.
The rate of thymidine incorporation into cells of Salmonella typhimurium growing in different media has been measured. In glucose-minimal medium, deoxyribonucleic acid (DNA) replication occurs during the first two-thirds of the division cycle; the final one-third of the division cycle was devoid of DNA replication. The measured doubling time of S. typhimurium in this medium is approximately 48 min, indicating that C (the time for a round of replication) and D (the time between termination and cell division) are approximately 32 and 16 min, respectively. At slower growth rates the pattern of replication is the same as glucose minimal medium. At faster growth rates the "gap" in DNA synthesis disappears. At rapid growth rates evidence for multiple forks is obtained.
已测定了在不同培养基中生长的鼠伤寒沙门氏菌细胞掺入胸苷的速率。在葡萄糖基本培养基中,脱氧核糖核酸(DNA)复制发生在分裂周期的前三分之二;分裂周期的最后三分之一没有DNA复制。在此培养基中测得的鼠伤寒沙门氏菌的倍增时间约为48分钟,这表明C(一轮复制的时间)和D(终止与细胞分裂之间的时间)分别约为32分钟和16分钟。在较慢的生长速率下,复制模式与葡萄糖基本培养基相同。在较快的生长速率下,DNA合成中的“间隙”消失。在快速生长速率下,可获得多复制叉的证据。