大肠杆菌B-r的染色体复制与分裂周期
Chromosome replication and the division cycle of Escherichia coli B-r.
作者信息
Kubitschek H E, Freedman M L
出版信息
J Bacteriol. 1971 Jul;107(1):95-9. doi: 10.1128/jb.107.1.95-99.1971.
Abstract
The average amount of deoxyribonucleic acid (DNA) per cell was measured in steady-state cultures of Escherichia coli B/r grown at 37 C in glucose-limited chemostats or in batch cultures in the exponential growth phase as maintained with one of several carbon sources. Within experimental errors, DNA content was dependent only on growth rate and independent of the type of culture, the carbon source, or the addition of growth factors. The amount of DNA per cell increased continuously with growth rate over the range of 0.02 to 3 divisions per hour. The data over the entire range of growth rates are in agreement with a constant time for a single replication point to traverse the entire genome, 47 min, and with cell division following 25 min after termination of replication. The measured amount of DNA per genome was 4.2 x 10(-15) g (or 2.5 x 10(9) daltons).
摘要
在37℃下，于葡萄糖受限的恒化器中培养的大肠杆菌B/r稳态培养物，或在以几种碳源之一维持的指数生长期分批培养物中，测量了每个细胞的脱氧核糖核酸（DNA）平均含量。在实验误差范围内，DNA含量仅取决于生长速率，而与培养类型、碳源或生长因子的添加无关。在每小时0.02至3次分裂的范围内，每个细胞的DNA量随生长速率持续增加。在整个生长速率范围内的数据与单个复制点遍历整个基因组所需的恒定时间（47分钟）一致，并且细胞分裂在复制终止后25分钟进行。每个基因组测得的DNA量为4.2×10⁻¹⁵克（或2.5×10⁹道尔顿）。
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本文引用的文献
1
MUTATION IN CONTINUOUS CULTURES. I. DEPENDENCE OF MUTATIONAL RESPONSE UPON GROWTH-LIMITING FACTORS.连续培养中的突变。I. 突变反应对生长限制因素的依赖性。
Mutat Res. 1964 Jul;106:113-20. doi: 10.1016/0027-5107(64)90013-2.
2
SEQUENTIAL REPLICATION OF THE BACILLUS SUBTILIS CHROMOSOME. 3. REGULATION OF INITIATION.枯草芽孢杆菌染色体的连续复制。3. 起始调控。
Proc Natl Acad Sci U S A. 1964 Oct;52(4):973-80. doi: 10.1073/pnas.52.4.973.
3
Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium.鼠伤寒沙门氏菌平衡生长期间细胞大小和化学成分对培养基及温度的依赖性。
J Gen Microbiol. 1958 Dec;19(3):592-606. doi: 10.1099/00221287-19-3-592.
4
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
5
DNA cycle of Myxococcus xanthus.黄色黏球菌的DNA循环
J Mol Biol. 1970 May 14;49(3):609-19. doi: 10.1016/0022-2836(70)90285-8.
6
Onset of DNA synthesis during the cell cycle in chemostat cultures.恒化器培养中细胞周期内DNA合成的起始
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611-7. doi: 10.1073/pnas.57.6.1611.
7
Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates.大肠杆菌中脱氧核糖核酸合成的调控：对生长速率的依赖性。
Biochim Biophys Acta. 1966 Jun 22;119(3):517-25. doi: 10.1016/0005-2787(66)90128-6.
8
Growth during the bacterial cell cycle: analysis of cell size distribution.细菌细胞周期中的生长：细胞大小分布分析
Biophys J. 1969 Jun;9(6):792-809. doi: 10.1016/S0006-3495(69)86418-0.
9
Synchronization of E. coli K 12 by membrane selection.
Biochem Biophys Res Commun. 1970 Oct 23;41(2):471-6. doi: 10.1016/0006-291x(70)90529-2.
10
Cytological studies of deoxyribonucleic acid replication in Escherichia coli 15T-: replication at slow growth rates and after a shift-up into rich medium.大肠杆菌15T-中脱氧核糖核酸复制的细胞学研究：在缓慢生长速率下以及转入丰富培养基后的复制
J Bacteriol. 1970 Oct;104(1):401-9. doi: 10.1128/jb.104.1.401-409.1970.
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