T7噬菌体DNA的注射顺序。
Order of injection of T7 bacteriophage DNA.
作者信息
Pao C C, Speyer J F
出版信息
J Virol. 1973 Jun;11(6):1024-6. doi: 10.1128/JVI.11.6.1024-1026.1973.
Abstract
The deoxyribonucleic acid (DNA) corresponding to the left-hand end of the genetic map is injected first in T7 phage infection. The order was deduced by use of marker rescue experiments to see which genes are still injected after the double-stranded DNA chain is broken by X rays. The treatment causes a selective loss of genes located on the right side of the map. Presumably, this corresponds to the tail end of normally injected DNA, and X rays cut off this end.
摘要
在T7噬菌体感染过程中，首先注入的是与遗传图谱左端相对应的脱氧核糖核酸（DNA）。通过标记拯救实验推断出这个顺序，以观察在双链DNA链被X射线打断后哪些基因仍被注入。这种处理导致位于图谱右侧的基因选择性丢失。据推测，这对应于正常注入DNA的末端，而X射线切断了这个末端。
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