丝心蛋白信使核糖核酸中与聚腺苷酸相邻的核苷酸序列。
The nucleotide sequence adjacent to poly(A) in silk fibroin messenger RNA.
作者信息
Hagenbüchle O, Krikeles M S, Sprague K U
出版信息
J Biol Chem. 1979 Aug 10;254(15):7157-62.
The 3'-terminal nucleotide sequence of fibroin mRNA has been determined. A cDNA transcript primed by oligo(dT) hybridized to the poly(A) segment of fibroin mRNA was used to determine the sequence immediately adjacent to poly(A). A specific primer complementary to this sequence then allowed synthesis of 5'-terminally homogeneous transcripts amenable to analysis by rapid sequencing methods. The 3' terminus of fibroin mRNA contains an unusually long oligo(U) stretch, as well as the sequence A2UA3 recently found in 3' non-coding regions of other eukaryotic mRNAs. It lacks the oligonucleotides characteristic of the repetitive coding region of fibroin mRNA.
已确定丝心蛋白mRNA的3'末端核苷酸序列。通过与丝心蛋白mRNA的聚腺苷酸（poly(A)）片段杂交的寡聚（dT）引发的cDNA转录本用于确定紧邻聚腺苷酸的序列。与该序列互补的特异性引物随后使得能够合成5'末端均一的转录本，便于通过快速测序方法进行分析。丝心蛋白mRNA的3'末端包含一段异常长的寡聚尿苷酸（oligo(U)）序列，以及最近在其他真核mRNA的3'非编码区发现的序列A2UA3。它缺乏丝心蛋白mRNA重复编码区的特征性寡核苷酸序列。
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