Biebricher C K, Orgel L E
Proc Natl Acad Sci U S A. 1973 Mar;70(3):934-8. doi: 10.1073/pnas.70.3.934.
We have selected from a mixture of random polynucleotides an RNA that is able to replicate in the presence of the DNA-dependent RNA polymerase of Escherichia coli, provided the medium contains Mn(2+) and ITP, in addition to ATP, CTP, and UTP. The RNA consists of a chain of about 125 (+/-25) nucleotides and appears to have a defined, nonrepetitive sequence.
我们从随机多核苷酸混合物中筛选出了一种RNA,只要培养基中除了含有ATP、CTP和UTP外,还含有Mn(2+)和ITP,这种RNA就能在大肠杆菌的DNA依赖性RNA聚合酶存在的情况下进行复制。该RNA由一条约125(±25)个核苷酸的链组成,并且似乎具有特定的、非重复的序列。