大肠杆菌L-阿拉伯糖操纵子的诱导动力学
Induction kinetics of the L-arabinose operon of Escherichia coli.
作者信息
Schleif R, Hess W, Finkelstein S, Ellis D
出版信息
J Bacteriol. 1973 Jul;115(1):9-14. doi: 10.1128/jb.115.1.9-14.1973.
Abstract
After addition of l-arabinose to growing Escherichia coli, the l-ribulokinase (EC 2.7.1.16) and l-arabinose isomerase (EC 5.3.1.4) first appear at about 0.7 and 1.4 min, respectively. These times are consistent with the distances of the genes from the ribonucleic acid polymerase initiation site in the operon. The kinetics of appearance of these enzymes as well as those of beta-galactosidase (EC 3.2.1.23) in the same strain are consistent with a peptide elongation rate of no less than 14 amino acids per second. A measurement of the average peptide elongation rate made by measuring the kinetics of radioactive amino acid appearance in completed polypeptides yielded a rate of about 12 amino acids per s. Convenient assays of the arabinose isomerase and ribulokinase are also given.
摘要
在向生长中的大肠杆菌添加L-阿拉伯糖后，L-核糖激酶（EC 2.7.1.16）和L-阿拉伯糖异构酶（EC 5.3.1.4）分别在约0.7分钟和1.4分钟首次出现。这些时间与操纵子中基因距核糖核酸聚合酶起始位点的距离一致。这些酶以及同一菌株中β-半乳糖苷酶（EC 3.2.1.23）出现的动力学与每秒不少于14个氨基酸的肽链延伸速率一致。通过测量完整多肽中放射性氨基酸出现的动力学来测定平均肽链延伸速率，结果得出约每秒12个氨基酸的速率。还给出了阿拉伯糖异构酶和核糖激酶的便捷检测方法。
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