通过大肠杆菌K-12中的阿拉伯糖转运系统进行乳糖渗透。
Lactose permeation via the arabinose transport system in Escherichia coli K-12.
作者信息
Messer A
出版信息
J Bacteriol. 1974 Oct;120(1):266-72. doi: 10.1128/jb.120.1.266-272.1974.
Abstract
This paper describes the isolation and characterization of a mutant of Escherichia coli that transports lactose and its analog thiomethylgalactoside via the arabinose permeation system. Unlike transport via the lactose permease, this transport is not inhibited by thiodigalactoside, but was inhibited by arabinose, xylose, and fucose. The site of the mutation was in the arabinose C gene and confers constitutivity on the entire arabinose operon. Furthermore, this transport was found in 24 independently isolated arabinose-constitutive strains, and in strains which had been induced with arabinose and then starved to remove all traces of it. It was therefore concluded that lactose and thiomethylgalactoside are low-affinity substrates of at least one component of the normal arabinose permeation system.
摘要
本文描述了一种大肠杆菌突变体的分离和特性，该突变体通过阿拉伯糖渗透系统转运乳糖及其类似物硫代甲基半乳糖苷。与通过乳糖通透酶的转运不同，这种转运不受硫代二半乳糖苷抑制，但受阿拉伯糖、木糖和岩藻糖抑制。突变位点位于阿拉伯糖C基因，赋予整个阿拉伯糖操纵子组成型表达。此外，在24个独立分离的阿拉伯糖组成型菌株以及用阿拉伯糖诱导后再饥饿以去除所有痕迹的菌株中发现了这种转运。因此得出结论，乳糖和硫代甲基半乳糖苷是正常阿拉伯糖渗透系统至少一种组分的低亲和力底物。
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