Vodkin M H, Fink G R
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1069-72. doi: 10.1073/pnas.70.4.1069.
A crude membrane fraction was isolated from both killer M(k) and isogenic nonkiller M(o) strains of yeast labeled with [(3)H]adenine. Nucleic acids were extracted from this fraction and centrifuged to equilibrium in an ethidium bromide-Cs(2)SO(4) solution. A peak of radioactivity coincident with a single fluorescent band was present in the membrane fraction isolated from cells of the killer strain but was absent from that isolated from nonkiller cells. This material has been characterized as double-stranded RNA by treatment with various nucleases and by chromatography on a cellulose CF-11 column. Sedimentation in a sucrose gradient indicates an S value of 8-10.
从用[³H]腺嘌呤标记的酵母杀伤型M(k)菌株和同基因非杀伤型M(o)菌株中分离出粗制膜组分。从该组分中提取核酸，并在溴化乙锭-Cs₂SO₄溶液中离心至平衡。在从杀伤型菌株细胞分离的膜组分中出现了一个与单一荧光带重合的放射性峰，而从非杀伤型细胞分离的膜组分中则没有。通过用各种核酸酶处理以及在纤维素CF-11柱上进行色谱分析，该物质已被鉴定为双链RNA。在蔗糖梯度中的沉降表明其S值为8-10。
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