Nitrogenase of Klebsiella pneumoniae: evidence for an adenosine triphosphate-induced association of the iron-sulphur protein.

The effect of various nucleotides on the Fe-containing component of nitrogenase of Klebsiella pneumoniae was investigated by ultracentrifugation and thiol-group reactivity towards 5,5'-dithiobis-(2-nitrobenzoate). In the absence of Na(2)S(2)O(4), ATP and ADP produced changes in sedimentation behaviour and thiol-group reactivity consistent with association of the protein.