Evans E E, Cushing J E, Evans M L
Infect Immun. 1973 Sep;8(3):355-9. doi: 10.1128/iai.8.3.355-359.1973.
This report continues comparative studies of inducible bactericidins of invertebrates. The bactericidin of the sipunculid worm Dendrostomum zostericolum Chamberlain was induced by intracoelomic injections of killed bacteria. With the assay system used, optimal results were obtained with 90 min of incubation at 20 to 35 C. Bactericidal titers in coelomic fluids from noninjected worms were either low or nonexistent. After injections of gram-negative bacteria, bactericidal titers of up to 1:1280 were reached within 7 days. Considerable individual variations were noted. Optimal stimulation was achieved with 4 x 10(8) bacteria per injection. The response was relatively nonspecific as measured by cross-immunization and absorption experiments. Injections of sterile sea water produced no response. Coelomic fluids could be inactivated by heating at 50 or 56 C for 20 min. Activity was not restored by a pool of unheated coelomic fluid from nonimmunized worms. The significance of these observations was discussed with reference to immunity of sipunculids and to comparative immunology.
本报告继续进行无脊椎动物诱导杀菌素的比较研究。通过向方格星虫Dendrostomum zostericolum Chamberlain的体腔内注射死菌来诱导其杀菌素。在所使用的检测系统中，在20至35℃孵育90分钟可获得最佳结果。未注射的蠕虫体腔液中的杀菌效价很低或不存在。注射革兰氏阴性菌后，7天内杀菌效价可达1:1280。观察到相当大的个体差异。每次注射4×10⁸个细菌可实现最佳刺激。通过交叉免疫和吸收实验测量，该反应相对非特异性。注射无菌海水无反应。体腔液在50或56℃加热20分钟可使其失活。未免疫蠕虫的未加热体腔液混合液不能恢复其活性。结合方格星虫的免疫和比较免疫学对这些观察结果的意义进行了讨论。
