Sakamoto H, Oda T
J Immunol Methods. 1979;26(4):325-35. doi: 10.1016/0022-1759(79)90178-9.
Sixty to 70% viability of lymphocytes in frozen tissue section was obtained by using dimethylsulphoxide-containing medium as a cryoprotective agent and by conditioning time and temperature for freezing, making sections on a cryostat and thawing. The aviable and dead cells were differentiated by trypan blue exclusion test on section. Lymphocytes in tissue sections showed spontaneous rosette formation with sheep erythrocytes (E rosette) with specific localization for each lymphoid organ.
通过使用含二甲亚砜的培养基作为冷冻保护剂，并对冷冻的时间和温度进行调控，在低温恒温器上切片和解冻，冷冻组织切片中的淋巴细胞存活率达到了60%至70%。通过对切片进行台盼蓝排斥试验来区分活细胞和死细胞。组织切片中的淋巴细胞与绵羊红细胞呈现自发玫瑰花结形成（E玫瑰花结），每个淋巴器官都有特定的定位。
