Lockhart M S, Comp P C, Taylor F B
J Lab Clin Med. 1979 Aug;94(2):285-94.
The role of platelets in clot lysis has been investigated functionally with the use of dPRP clots formed at 4 degrees and shifted to 37 degrees. Clots handled in this manner lysed in 6 hr (+/- 1 hr), whereas clots formed at 4 degrees or at 37 degrees and held at those temperatures, or clots formed from dPPP did not lyse in less than 20 hr. dPRP clots having the shorter (6 hr) lysis time released 14C-5-HT at the time of the temperature shift. Preincubation of dPRP with antimycin A and 2-deoxy-D-glucose before addition of thrombin prolonged the clot lysis to 26 hr and inhibited release of 14C-5-HT at the time of the temperature shift. These studies demonstrate that metabolically active platelets are required to mediate the optimal clot lysis seen in the 4 degrees to 37 degrees system and that they continue to function (i.e., take up and release 5-hydroxytryptamine) after they have been incorporated into a clot. Thus the dPRP clot lysis system provides a model by which the timing and sequence of the interaction of metabolically active platelets with the fibrin framework of the formed clot can be studied.
通过使用在4℃形成并转移至37℃的富血小板血浆（dPRP）凝块，对血小板在凝块溶解中的作用进行了功能研究。以这种方式处理的凝块在6小时（±1小时）内溶解，而在4℃或37℃形成并保持在这些温度下的凝块，或由贫血小板血浆（dPPP）形成的凝块在少于20小时内不会溶解。具有较短（6小时）溶解时间的dPRP凝块在温度转移时释放14C - 5 - 羟色胺（5 - HT）。在加入凝血酶之前，用抗霉素A和2 - 脱氧 - D - 葡萄糖对dPRP进行预孵育，可将凝块溶解时间延长至26小时，并抑制温度转移时14C - 5 - HT的释放。这些研究表明，需要代谢活跃的血小板来介导在4℃至37℃系统中看到的最佳凝块溶解，并且它们在被整合到凝块中后仍继续发挥作用（即摄取和释放5 - 羟色胺）。因此，dPRP凝块溶解系统提供了一个模型，通过该模型可以研究代谢活跃的血小板与形成的凝块的纤维蛋白框架相互作用的时间和顺序。
