Gilliam J M, Berkus V, Taylor F B
Am J Pathol. 1979 Jul;96(1):35-50.
Platelets in a dilute platelet-rich plasma clot formed by the addition of thrombin at 4 C do not lose their alpha-granules or develop platelet-fibrin interconnections. These clots do not retract or lyse. If after clot formation for 30 minutes the clots are transferred to 37 C, the alpha-granules then disappear from the platelet, extensive platelet-fibrin interconnections develop, and the clots retract and lyse. When the dilute plasma clots, however, are formed at 37 C, alpha-granules are quickly lost, few platelet-fibrin interconnections develop, and the clots do not retract or lyse. Deoxyglucose and antimycin, when added before thrombin at 4 C, prevent the loss of alpha-granules and development of platelet-fibrin interconnections after transfer of the clot to 37 C. These inhibited platelets did not retract or lyse. From the results reported here we conclude that the development of platelet-fibrin interconnections as well as the retraction and lysis of the clot occur in conjunction with the release of alpha-granules from the platelet. It may be necessary for this release to occur after the fibrin network is completed, since release of the alpha-granules in the 37 C clot before clot formation did not result in formation of many platelet-fibrin interconnections or in clot retraction or lysis.
在4℃下通过添加凝血酶形成的稀释富血小板血浆凝块中的血小板不会失去其α-颗粒，也不会形成血小板-纤维蛋白连接。这些凝块不会收缩或溶解。如果在凝块形成30分钟后将凝块转移到37℃，那么α-颗粒随后会从血小板中消失，广泛的血小板-纤维蛋白连接会形成，并且凝块会收缩和溶解。然而，当稀释血浆凝块在37℃形成时，α-颗粒会迅速丢失，很少形成血小板-纤维蛋白连接，并且凝块不会收缩或溶解。在4℃下，脱氧葡萄糖和抗霉素在凝血酶之前添加时，可防止凝块转移到37℃后α-颗粒的丢失和血小板-纤维蛋白连接的形成。这些受抑制的血小板不会收缩或溶解。根据此处报道的结果，我们得出结论，血小板-纤维蛋白连接的形成以及凝块的收缩和溶解与血小板中α-颗粒的释放同时发生。由于在凝块形成前在37℃凝块中α-颗粒的释放并未导致形成许多血小板-纤维蛋白连接或凝块收缩或溶解，因此这种释放在纤维蛋白网络完成后发生可能是必要的。
