质粒脱氧核糖核酸的分离通用方法。
General method for the isolation of plasmid deoxyribonucleic acid.
作者信息
Guerry P, LeBlanc D J, Falkow S
出版信息
J Bacteriol. 1973 Nov;116(2):1064-6. doi: 10.1128/jb.116.2.1064-1066.1973.
Abstract
Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 10(6) to 65 x 10(6) daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in the presence of sodium lauryl sulfate and a high concentration of NaCl.
摘要
通过在十二烷基硫酸钠和高浓度氯化钠存在的情况下，优先沉淀高分子量的染色体DNA，可以从染色体DNA中分离出分子量范围为5×10⁶至65×10⁶道尔顿的质粒脱氧核糖核酸（DNA）。
相似文献
1
General method for the isolation of plasmid deoxyribonucleic acid.质粒脱氧核糖核酸的分离通用方法。
J Bacteriol. 1973 Nov;116(2):1064-6. doi: 10.1128/jb.116.2.1064-1066.1973.
2
Extrachromosomal elements in group N streptococci.N群链球菌中的染色体外元件
J Bacteriol. 1974 Mar;117(3):1149-52. doi: 10.1128/jb.117.3.1149-1152.1974.
3
Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae.淋病奈瑟菌质粒脱氧核糖核酸的特性分析。
Infect Immun. 1974 Oct;10(4):712-7. doi: 10.1128/iai.10.4.712-717.1974.
4
Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.通过染料浮力密度离心法从细菌裂解物中获得更准确的共价闭合环状质粒与染色体比率的方法。
J Bacteriol. 1977 Apr;130(1):148-53. doi: 10.1128/jb.130.1.148-153.1977.
5
Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).从天蓝色链霉菌A3(2)中分离共价闭合环状脱氧核糖核酸。
J Bacteriol. 1975 Feb;121(2):416-21. doi: 10.1128/jb.121.2.416-421.1975.
6
Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides.赋予链霉素和磺胺类药物连锁抗性的质粒的脱氧核糖核酸分子量及同源性比较
J Bacteriol. 1974 Nov;120(2):618-30. doi: 10.1128/jb.120.2.618-630.1974.
7
Isolation of minicircular deoxyribonucleic acids from wild strains of Escherichia coli and their relationship to other bacterial plasmids.从大肠杆菌野生菌株中分离微小环状脱氧核糖核酸及其与其他细菌质粒的关系。
J Bacteriol. 1972 Sep;111(3):696-704. doi: 10.1128/jb.111.3.696-704.1972.
8
Isolation of cryptic plasmid deoxyribonucleic acid from Kanagawa-positive strains of Vibrio parahaemolyticus.从副溶血性弧菌神奈川阳性菌株中分离隐蔽性质粒脱氧核糖核酸。
Infect Immun. 1977 Apr;16(1):328-34. doi: 10.1128/iai.16.1.328-334.1977.
9
Determination of the length of the V colicinogenic factor and a derivative fertility factor.V 型大肠杆菌素生成因子及一种衍生的致育因子长度的测定
J Bacteriol. 1973 Dec;116(3):1474-6. doi: 10.1128/jb.116.3.1474-1476.1973.
10
Asymmetry and extent of in vivo transcripition of R-plasmid deoxyribonucleic acid in Escherichia coli.R质粒脱氧核糖核酸在大肠杆菌体内转录的不对称性及程度
J Bacteriol. 1974 Dec;120(3):1274-8. doi: 10.1128/jb.120.3.1274-1278.1974.
引用本文的文献
1
Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.细菌拟核是一个谜团，被包裹在一个谜中，又被包含在另一个谜里。
J Bacteriol. 2024 Mar 21;206(3):e0021123. doi: 10.1128/jb.00211-23. Epub 2024 Feb 15.
2
Electrochemical DNA Sensor Designed Using the Pencil Graphite Electrode to Detect Listeria monocytogenes.基于铅笔石墨电极设计的用于检测李斯特菌的电化学 DNA 传感器。
Appl Biochem Biotechnol. 2024 Jul;196(7):4679-4698. doi: 10.1007/s12010-023-04732-2. Epub 2023 Sep 29.
3
Catastrophic chromosome fragmentation probes the nucleoid structure and dynamics in Escherichia coli.灾难性染色体碎片化探测大肠杆菌中核区结构和动力学。
Nucleic Acids Res. 2022 Oct 28;50(19):11013-11027. doi: 10.1093/nar/gkac865.
4
Conserved regions in the T-DNA of different Agrobacterium rhizogenes root-inducing plasmids.不同发根农杆菌 T-DNA 中的保守区。
Plant Mol Biol. 1987 Jan;9(1):19-26. doi: 10.1007/BF00017983.
5
Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeast.在酵母中构建新的酵母载体并克隆肺炎克雷伯氏菌的 nif（氮固定）基因簇。
Curr Genet. 1981 Jul;3(3):173-80. doi: 10.1007/BF00429819.
6
2 μm plasmid copy number in different yeast strains and repartition of endogenous and 2 μm chimeric plasmids in transformed strains.不同酵母菌株中的 2 μm 质粒拷贝数和转化菌株中内源性和 2 μm 嵌合质粒的重新分配。
Curr Genet. 1980 Apr;1(3):219-28. doi: 10.1007/BF00390947.
7
Cloning by genetic complementation and restriction mapping of the yeast HEM1 gene coding for 5-aminolevulinate synthase.通过遗传互补和限制图谱分析克隆编码 5-氨基乙酰丙酸合酶的酵母 HEM1 基因。
Curr Genet. 1984 Jul;8(5):327-31. doi: 10.1007/BF00419820.
8
Chromosomal DNA sequences from Ustilago maydis promote autonomous replication of plasmids in Saccharomyces cerevisiae.玉米黑粉菌的染色体 DNA 序列可促进酵母中质粒的自主复制。
Curr Genet. 1983 Mar;7(1):79-84. doi: 10.1007/BF00365685.
9
A one-step miniprep for the isolation of plasmid DNA and lambda phage particles.一步法小量抽提试剂盒，用于质粒 DNA 和 λ噬菌体颗粒的分离。
PLoS One. 2011;6(8):e23457. doi: 10.1371/journal.pone.0023457. Epub 2011 Aug 15.
10
Frequency-Dependent Selection for Plasmid-Containing Cells of ESCHERICHIA COLI.大肠杆菌含质粒细胞的频率依赖性选择。
Genetics. 1979 Apr;91(4):627-37. doi: 10.1093/genetics/91.4.627.
本文引用的文献
1
Membrane association by bacteriophage lambda-DNA: possible direct role of regulator gene N.噬菌体λ-DNA与膜的结合：调节基因N的可能直接作用
Nature. 1969 Sep 20;223(5212):1239-42. doi: 10.1038/2231239a0.
2
The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation.耐药病原菌的问题。接合后R因子DNA在大肠杆菌K-12中的复制。
Ann N Y Acad Sci. 1971 Jun 11;182:153-71. doi: 10.1111/j.1749-6632.1971.tb30654.x.
3
Specific labeling and physical characterization of R-factor deoxyribonucleic acid in Escherichia coli.大肠杆菌中R因子脱氧核糖核酸的特异性标记及物理特性研究
J Bacteriol. 1970 Oct;104(1):331-9. doi: 10.1128/jb.104.1.331-339.1970.
4
Multiple molecular species of circular R-factor DNA isolated from Escherichia coli.从大肠杆菌中分离出的环状R因子DNA的多种分子种类。
Nature. 1969 Dec 27;224(5226):1273-7. doi: 10.1038/2241273a0.
5
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物：纯化及诱导转化为开放环状DNA形式
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66. doi: 10.1073/pnas.62.4.1159.
6
Studies on Escherichia coli sex factors. I. Specific labeling of F'Lac DNA.大肠杆菌性因子的研究。I. F'Lac DNA的特异性标记
J Mol Biol. 1968 Feb 28;32(1):15-23. doi: 10.1016/0022-2836(68)90141-1.
7
The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.闭环DNA与嵌入染料的相互作用。I. 存在和不存在染料时SV40 DNA的超螺旋密度。
J Mol Biol. 1968 Apr 14;33(1):141-71. doi: 10.1016/0022-2836(68)90286-6.
8
Selective extraction of polyoma DNA from infected mouse cell cultures.从受感染的小鼠细胞培养物中选择性提取多瘤病毒DNA。
J Mol Biol. 1967 Jun 14;26(2):365-9. doi: 10.1016/0022-2836(67)90307-5.
Zotero 插件