美利奴羊毛还原态高硫蛋白的研究。蛋白质SCMKB - 3A3的氨基酸序列。
Studies on the high-sulphur proteins of reduced Merino wool. Amino acid sequence of protein SCMKB-3A3.
作者信息
Swart L S, Haylett T
出版信息
Biochem J. 1973 Aug;133(4):641-54. doi: 10.1042/bj1330641.
Abstract
The complete amino acid sequences of wool protein SCMKB-IIIA3 (131 residues) and a minor component SCMKB-IIIA3A (130 residues) have been determined. The proteins are mutually homologous and have free threonine as the N-terminal residue and carboxymethylcysteine as the C-terminus. The peptides used for the sequence work were obtained by trypsin, thermolysin, pepsin and chymotrypsin digestions and were fractionated by chromatography on DEAE-cellulose, gel filtration on Sephadex G-25 and G-50, paper chromatography and electrophoresis. The Edman degradation method (employing both the Beckman Sequencer and a non-automatic procedure) was used to obtain the sequences of the peptides.
摘要
羊毛蛋白SCMKB-IIIA3（131个残基）和一种次要成分SCMKB-IIIA3A（130个残基）的完整氨基酸序列已被确定。这两种蛋白质相互同源，N端残基为游离苏氨酸，C端为羧甲基半胱氨酸。用于序列分析的肽段通过胰蛋白酶、嗜热菌蛋白酶、胃蛋白酶和胰凝乳蛋白酶消化获得，并通过DEAE-纤维素柱色谱、Sephadex G-25和G-50凝胶过滤、纸色谱和电泳进行分离。采用埃德曼降解法（同时使用贝克曼测序仪和非自动方法）来获得肽段的序列。
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