美利奴羊毛还原后高硫蛋白的研究。蛋白质SCMKB - 3B 3的氨基酸序列。
Studies on the high-sulphur proteins of reduced merino wool. Amino acid sequence of protein SCMKB-3B 3 .
作者信息
Haylett T, Swart L S, Parris D
出版信息
Biochem J. 1971 Jun;123(2):191-200. doi: 10.1042/bj1230191.
Abstract
The complete amino acid sequence of wool protein SCMKB-IIIB3 was determined. The peptides used for the sequence work were obtained by peptic and thermolysin digestions and were fractionated by chromatography on DEAE-cellulose, paper chromatography and electrophoresis. The peptides were analysed by dansyl-Edman degradation, mass spectrometry and tritium-labelling of C-terminal residues. The protein consists of 98 residues and has acetylalanine as N-terminal residue and carboxymethylcysteine as C-terminus. It is homologous with protein SCMKB-IIIB2 (Haylett & Swart, 1969). A salient feature of the sequence of protein SCMKB-IIIB3 is three consecutive cysteine residues.
摘要
羊毛蛋白SCMKB-IIIB3的完整氨基酸序列已被确定。用于序列分析的肽段是通过胃蛋白酶和嗜热菌蛋白酶消化获得的，并通过DEAE-纤维素柱色谱、纸色谱和电泳进行分离。这些肽段通过丹磺酰-埃德曼降解、质谱分析以及C末端残基的氚标记进行分析。该蛋白质由98个残基组成，N末端残基为乙酰丙氨酸，C末端为羧甲基半胱氨酸。它与蛋白质SCMKB-IIIB2同源（Haylett & Swart，1969）。蛋白质SCMKB-IIIB3序列的一个显著特征是有三个连续的半胱氨酸残基。
相似文献
1
Studies on the high-sulphur proteins of reduced merino wool. Amino acid sequence of protein SCMKB-3B 3 .美利奴羊毛还原后高硫蛋白的研究。蛋白质SCMKB - 3B 3的氨基酸序列。
Biochem J. 1971 Jun;123(2):191-200. doi: 10.1042/bj1230191.
2
Studies on the high-sulphur proteins of reduced merino wool. Amino acid sequence of protein SCMKB-3B 4 .美利奴细毛还原后高硫蛋白的研究。蛋白质SCMKB - 3B 4的氨基酸序列
Biochem J. 1971 Jun;123(2):201-10. doi: 10.1042/bj1230201.
3
Studies on the high-sulphur proteins of reduced Merino wool. Amino acid sequence of protein SCMKB-3A3.美利奴羊毛还原态高硫蛋白的研究。蛋白质SCMKB - 3A3的氨基酸序列。
Biochem J. 1973 Aug;133(4):641-54. doi: 10.1042/bj1330641.
4
Studies on the high-sulphur proteins of reduced mohair. The isolation and amino acid sequence of protein scmkb-m1.2.还原马海毛的高硫蛋白研究。蛋白质scmkb - m1.2的分离及氨基酸序列
Biochem J. 1975 Mar;145(3):459-67. doi: 10.1042/bj1450459.
5
The sequence of SCMK-B2B, a high-sulfur protein from wool keratin.羊毛角蛋白中的一种高硫蛋白SCMK-B2B的序列。
J Biol Chem. 1972 Jun 25;247(12):3900-9.
6
The amino acid sequence of protein SCMK-B2C from the high-sulphur fraction of wool keratin.羊毛角蛋白高硫组分中蛋白质SCMK-B2C的氨基酸序列。
Biochem J. 1972 Aug;128(5):1229-39. doi: 10.1042/bj1281229.
7
Chymotryptic inhibitor I from potatoes. The amino acid sequence of subunit A.来自土豆的胰凝乳蛋白酶抑制剂I。亚基A的氨基酸序列。
Biochem J. 1974 Jan;137(1):101-12. doi: 10.1042/bj1370101.
8
The preparation and properties of a group of proteins from the high-sulphur fraction of wool.羊毛高硫组分中一组蛋白质的制备及其性质
Biochem J. 1972 Jul;128(4):859-67. doi: 10.1042/bj1280859.
9
Proteins of normal hair and of cystine-deficient hair from mentally retarded siblings.来自智障同胞的正常头发和胱氨酸缺乏头发的蛋白质。
Biochem J. 1971 May;122(4):433-44. doi: 10.1042/bj1220433.
10
The amino acid sequence of protein SCMK-B2A from the high-sulphur fraction of wool keratin.羊毛角蛋白高硫组分中蛋白质SCMK-B2A的氨基酸序列。
Biochem J. 1972 Dec;130(3):833-45. doi: 10.1042/bj1300833.
引用本文的文献
1
Wool Keratin-Associated Protein Genes in Sheep-A Review.绵羊中与羊毛角蛋白相关的蛋白质基因——综述
Genes (Basel). 2016 May 28;7(6):24. doi: 10.3390/genes7060024.
2
Dipeptide frequencies in proteins and the CpG deficiency in vertebrate DNA.蛋白质中的二肽频率与脊椎动物 DNA 中的 CpG 缺乏。
J Mol Evol. 1972 Dec;1(4):315-25. doi: 10.1007/BF01653960.
3
An updated nomenclature for keratin-associated proteins (KAPs).角蛋白相关蛋白（KAPs）的最新命名法。
Int J Biol Sci. 2012;8(2):258-64. doi: 10.7150/ijbs.3278. Epub 2012 Jan 12.
4
Studies on the high-sulphur proteins of reduced merino wool. Amino acid sequence of protein SCMKB-3B 4 .美利奴细毛还原后高硫蛋白的研究。蛋白质SCMKB - 3B 4的氨基酸序列
Biochem J. 1971 Jun;123(2):201-10. doi: 10.1042/bj1230201.
5
The amino acid sequence of protein SCMK-B2C from the high-sulphur fraction of wool keratin.羊毛角蛋白高硫组分中蛋白质SCMK-B2C的氨基酸序列。
Biochem J. 1972 Aug;128(5):1229-39. doi: 10.1042/bj1281229.
6
De novo origin of periodic proteins.周期性蛋白质的从头起源。
J Mol Evol. 1972 Dec 29;2(1):17-27. doi: 10.1007/BF01653939.
7
The preparation and properties of a group of proteins from the high-sulphur fraction of wool.羊毛高硫组分中一组蛋白质的制备及其性质
Biochem J. 1972 Jul;128(4):859-67. doi: 10.1042/bj1280859.
8
Studies on the high-sulphur proteins of reduced Merino wool. Amino acid sequence of protein SCMKB-3A3.美利奴羊毛还原态高硫蛋白的研究。蛋白质SCMKB - 3A3的氨基酸序列。
Biochem J. 1973 Aug;133(4):641-54. doi: 10.1042/bj1330641.
9
The reactivity of the disulphide bonds of wool.羊毛中二硫键的反应活性。
Biochem J. 1974 Jun;139(3):515-23. doi: 10.1042/bj1390515.
10
Studies on the high-sulphur proteins of reduced mohair. The isolation and amino acid sequence of protein scmkb-m1.2.还原马海毛的高硫蛋白研究。蛋白质scmkb - m1.2的分离及氨基酸序列
Biochem J. 1975 Mar;145(3):459-67. doi: 10.1042/bj1450459.
本文引用的文献
1
CHARACTERIZATION OF LAMPREY FIBRINOPEPTIDES.七鳃鳗纤维蛋白肽的特性分析
Biochem J. 1965 Mar;94(3):742-50. doi: 10.1042/bj0940742.
2
Halogenation of tyrosine during acid hydrolysis.酸水解过程中酪氨酸的卤化作用。
Biochim Biophys Acta. 1963 May 14;71:468-71. doi: 10.1016/0006-3002(63)91108-9.
3
The complete enzymic hydrolysis of proteins.蛋白质的完全酶解。
J Biol Chem. 1962 Feb;237:389-96.
4
A protein sequenator.蛋白质测序仪。
Eur J Biochem. 1967 Mar;1(1):80-91. doi: 10.1007/978-3-662-25813-2_14.
5
Thin-layer chromatographic separation of DNS-amino acids.DNS-氨基酸的薄层色谱分离
Anal Biochem. 1966 Mar;14(3):429-33. doi: 10.1016/0003-2697(66)90285-5.
6
Combinations of specific color reactions useful in the peptide mapping technique.在肽图谱技术中有用的特定颜色反应组合。
Biochim Biophys Acta. 1965 Sep 13;107(2):386-8. doi: 10.1016/0304-4165(65)90147-9.
7
Sequence analysis of microgram amounts of peptides by mass spectrometry.通过质谱法对微克量的肽进行序列分析。
Anal Biochem. 1970 Mar;34:286-91. doi: 10.1016/0003-2697(70)90109-0.
8
Mass spectrometric determination of the amino acid sequence in peptides: desulfurization of sulfur-containing peptides.肽中氨基酸序列的质谱测定：含硫肽的脱硫
Experientia. 1969 Sep 15;25(9):913-4. doi: 10.1007/BF01898058.
Zotero 插件