Haylett T, Swart L S, Parris D
Biochem J. 1971 Jun;123(2):191-200. doi: 10.1042/bj1230191.
The complete amino acid sequence of wool protein SCMKB-IIIB3 was determined. The peptides used for the sequence work were obtained by peptic and thermolysin digestions and were fractionated by chromatography on DEAE-cellulose, paper chromatography and electrophoresis. The peptides were analysed by dansyl-Edman degradation, mass spectrometry and tritium-labelling of C-terminal residues. The protein consists of 98 residues and has acetylalanine as N-terminal residue and carboxymethylcysteine as C-terminus. It is homologous with protein SCMKB-IIIB2 (Haylett & Swart, 1969). A salient feature of the sequence of protein SCMKB-IIIB3 is three consecutive cysteine residues.
羊毛蛋白SCMKB-IIIB3的完整氨基酸序列已被确定。用于序列分析的肽段是通过胃蛋白酶和嗜热菌蛋白酶消化获得的，并通过DEAE-纤维素柱色谱、纸色谱和电泳进行分离。这些肽段通过丹磺酰-埃德曼降解、质谱分析以及C末端残基的氚标记进行分析。该蛋白质由98个残基组成，N末端残基为乙酰丙氨酸，C末端为羧甲基半胱氨酸。它与蛋白质SCMKB-IIIB2同源（Haylett & Swart，1969）。蛋白质SCMKB-IIIB3序列的一个显著特征是有三个连续的半胱氨酸残基。
