Brazenor G A, Davies D J
Clin Exp Immunol. 1973 Oct;15(2):303-7.
Isoantibodies which react strongly and specifically with donor lymphocytes have been demonstrated by membrane immunofluorescence in the sera of mice and rabbits bearing first and second set allografts of skin. Changes in the titre of these antibodies closely paralleled the course of the rejection reaction. With other types of cells the immunofluorescent staining was less intense and the reactions were less consistent. Staining was completely inhibited by serum absorption with spleen, lymph node or thymus, but myocardium, aorta or tendon had no specific absorbing effect. The antibodies detected are probably directed against histocompatibility antigens.
通过膜免疫荧光法已证实在接受首次和第二次皮肤同种异体移植的小鼠和兔子血清中存在能与供体淋巴细胞发生强烈且特异性反应的同种抗体。这些抗体效价的变化与排斥反应的进程密切平行。对于其他类型的细胞，免疫荧光染色强度较低且反应不太一致。用脾脏、淋巴结或胸腺进行血清吸收可完全抑制染色，但心肌、主动脉或肌腱没有特异性吸收作用。检测到的抗体可能针对组织相容性抗原。
