Stendahl O, Magnusson K E, Tagesson C, Cunningham R, Edebo L
Infect Immun. 1973 Apr;7(4):573-7. doi: 10.1128/iai.7.4.573-577.1973.
An aqueous, two-polymer phase system was employed in an attempt to separate and characterize a series of R mutants, derived from Salmonella typhimurium 395 MS, with differing lengths of lipopolysaccharide chains on their surfaces. R mutants with varying degrees of virulence and phagocytic resistance were unresolved in this system. However, the smooth MS bacteria were clearly separated from the rough cells and showed a high affinity for the polyethylene glycol-rich top phase. A uridine 5'-diphosphate-gal-4-epimeraseless mutant, phenotypically in R or S form depending on the growth medium, partitioned as the R mutants and S bacteria, respectively. These results demonstrate the great influence of long polysaccharide chains on the physicochemical properties of the cell surface.
采用水相双聚合物相系统试图分离和表征一系列源自鼠伤寒沙门氏菌395 MS的R突变体，这些突变体表面脂多糖链长度不同。在该系统中，具有不同毒力和吞噬抗性的R突变体无法区分。然而，光滑的MS细菌与粗糙细胞明显分离，并对富含聚乙二醇的上相表现出高亲和力。一个尿苷5'-二磷酸-半乳糖-4-表异构酶缺失突变体，根据生长培养基的不同，表型分别为R型或S型，分别像R突变体和S细菌一样进行分配。这些结果证明了长多糖链对细胞表面物理化学性质的巨大影响。
