通过在水相双聚合物体系中的逆流分配法对鼠伤寒沙门氏菌突变体进行表征。
Characterization of mutants of Salmonella typhimurium by counter-current distribution in an aqueous two-polymer phase system.
作者信息
Stendahl O, Magnusson K E, Tagesson C, Cunningham R, Edebo L
出版信息
Infect Immun. 1973 Apr;7(4):573-7. doi: 10.1128/iai.7.4.573-577.1973.
Abstract
An aqueous, two-polymer phase system was employed in an attempt to separate and characterize a series of R mutants, derived from Salmonella typhimurium 395 MS, with differing lengths of lipopolysaccharide chains on their surfaces. R mutants with varying degrees of virulence and phagocytic resistance were unresolved in this system. However, the smooth MS bacteria were clearly separated from the rough cells and showed a high affinity for the polyethylene glycol-rich top phase. A uridine 5'-diphosphate-gal-4-epimeraseless mutant, phenotypically in R or S form depending on the growth medium, partitioned as the R mutants and S bacteria, respectively. These results demonstrate the great influence of long polysaccharide chains on the physicochemical properties of the cell surface.
摘要
采用水相双聚合物相系统试图分离和表征一系列源自鼠伤寒沙门氏菌395 MS的R突变体，这些突变体表面脂多糖链长度不同。在该系统中，具有不同毒力和吞噬抗性的R突变体无法区分。然而，光滑的MS细菌与粗糙细胞明显分离，并对富含聚乙二醇的上相表现出高亲和力。一个尿苷5'-二磷酸-半乳糖-4-表异构酶缺失突变体，根据生长培养基的不同，表型分别为R型或S型，分别像R突变体和S细菌一样进行分配。这些结果证明了长多糖链对细胞表面物理化学性质的巨大影响。
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Role of cell wall structure of salmonella in the interaction with phagocytes.沙门氏菌细胞壁结构在与吞噬细胞相互作用中的作用。
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Counter-current distribution of cells.细胞的逆流分布
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Separation of lymphocytes and polymorphonuclear leukocytes by countercurrent distribution in aqueous two-polymer phase systems.在双聚合物水相体系中通过逆流分配法分离淋巴细胞和多形核白细胞。
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Separation of bacteriophage phiX174 mutants by countercurrent distribution in a two-polymer acqueous phase system.在双聚合物水相体系中通过逆流分配法分离噬菌体φX174突变体
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Virulence and immunogenicity of mutant strains of Salmonella typhimurium.鼠伤寒沙门氏菌突变株的毒力和免疫原性。
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Bacteriophage receptor development and synthesis of O-specific side chains after addition of D-galactose to the uridine diphosphate-galactose-4-epimeraseless mutant Salmonella typhimurium LT2-M1.向尿苷二磷酸半乳糖-4-表异构酶缺陷型鼠伤寒沙门氏菌LT2-M1添加D-半乳糖后噬菌体受体的发育及O-特异性侧链的合成。
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