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使用冻结前沿技术测定生物细胞的表面张力。

Determination of the surface tension of biological cells using the freezing front technique.

作者信息

Spelt J K, Absolom D R, Zingg W, van Oss C J, Neumann A W

出版信息

Cell Biophys. 1982 Jun-Sep;4(2-3):117-31. doi: 10.1007/BF02918308.

DOI:10.1007/BF02918308
PMID:6181879
Abstract

The freezing front technique for solid surface tension measurements was used to obtain the surface tensions of glutaraldehyde-fixed human erythrocytes, and fresh human lymphocytes and granulocytes in aqueous media. The results agree well with the values obtained by other methods and indicate that the freezing front technique is sufficiently sensitive to detect small differences (of the order of 0.1 ergs/cm2) in surface tension. This property, along with a number of applications for which it is uniquely suited, makes the freezing front technique an important new approach to the measurement of the surface tensions of biological cells and of small particles in general.

摘要

采用凝固前沿技术测量固体表面张力,以获取戊二醛固定的人红细胞、新鲜人淋巴细胞和粒细胞在水性介质中的表面张力。结果与其他方法得到的值吻合良好,表明凝固前沿技术足够灵敏,能够检测到表面张力的微小差异(约0.1尔格/平方厘米)。这一特性以及它特别适合的一些应用,使凝固前沿技术成为测量生物细胞和一般小颗粒表面张力的一种重要新方法。

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1
Determination of the surface tension of biological cells using the freezing front technique.使用冻结前沿技术测定生物细胞的表面张力。
Cell Biophys. 1982 Jun-Sep;4(2-3):117-31. doi: 10.1007/BF02918308.
2
Determination of the surface tension of various species of erythrocytes by means of the solidification front technique.通过凝固前沿技术测定不同种类红细胞的表面张力。
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本文引用的文献

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Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.细胞化学与电子显微镜术。醛类固定对细胞超微结构及酶活性的保存作用。
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Determination of the surface tension of various species of erythrocytes by means of the solidification front technique.通过凝固前沿技术测定不同种类红细胞的表面张力。
Cell Biophys. 1985 Dec;7(4):267-81. doi: 10.1007/BF02789438.
血小板从悬浮液黏附到各种底物的动力学和热力学方面。
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Elution of human granulocytes from nylon fibers by means of repulsive van der Waals forces.
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Platelet adhesion to smooth and rough hydrophobic and hydrophilic surfaces under conditions of static exposure and laminar flow.在静态暴露和层流条件下血小板对光滑及粗糙疏水和亲水表面的黏附。
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Phagocytosis as a surface phenomenon: opsonization by aspecific adsorption of IgG as a function of bacterial hydrophobicity.作为一种表面现象的吞噬作用:IgG 通过特异性吸附的调理作用与细菌疏水性的关系。
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Determination of the Surface tension of proteins. I. Surface tension of native serum proteins in aqueous media.
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Surface thermodynamics of normal and pathological human granulocytes.
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Cell surface energy, contact angles and phase partition. II. Bacterial cells in biphasic aqueous mixtures.细胞表面能、接触角与相分配。II. 双相水性混合物中的细菌细胞。
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Cell surface energy, contact angles and phase partition. I. Lymphocytic cell lines in biphasic aqueous mixtures.细胞表面能、接触角与相分配。I. 双相水性混合物中的淋巴细胞系
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