Romball C G, Weigle W O
J Exp Med. 1973 Dec 1;138(6):1426-42. doi: 10.1084/jem.138.6.1426.
After a single intravenous injection of rabbits with aggregated HuIgG, IgM- and IgG-plaque-forming cells (PFC) in both the spleens and peripheral blood of rabbits peaked 5, 13, and 21 days after injection, while almost no PFC could be detected on days 8 and 16. The available data suggest that the secondary peaks of PFC (days 13 and 21) resulted from stimulation of memory cells by persisting antigen that was localized in the germinal centers in the spleen. No such persistence of antigen occurred in the lymph nodes, and these lymphoid tissues did not exhibit secondary peaks of PFC. The identical kinetic patterns for IgM- and IgG-PFC indicate that the major portion of IgG-PFC did not result from IgM-secreting cells switching to IgG synthesis and secretion. The present data suggest that the antibody produced and present at the site of interaction between committed cells and antigen is responsible for the regulation of antibody synthesis to persisting antigens. Possible cellular events involved in both the regulation and an apparent synchronous appearance of antibody producing cells in the spleens of rabbits were presented.
给兔子单次静脉注射聚合人IgG后，兔子脾脏和外周血中的IgM和IgG斑块形成细胞（PFC）在注射后第5、13和21天达到峰值，而在第8天和第16天几乎检测不到PFC。现有数据表明，PFC的二次峰值（第13天和第21天）是由位于脾脏生发中心的持续存在的抗原刺激记忆细胞所致。淋巴结中未出现这种抗原的持续存在，并且这些淋巴组织未表现出PFC的二次峰值。IgM和IgG - PFC相同的动力学模式表明，大部分IgG - PFC并非由分泌IgM的细胞转换为合成和分泌IgG所致。目前的数据表明，在定型细胞与抗原相互作用部位产生并存在的抗体负责对持续存在的抗原的抗体合成调节。本文还介绍了可能参与兔子脾脏中抗体产生细胞调节及明显同步出现的细胞事件。
